“Our mission is to protect and promote animal and human health through early detection and monitoring of animal diseases”
The University Diagnostic Lab (UDL) of the University of Veterinary & Animal Sciences, (UVAS) Lahore is a national leader in providing rapid diagnosis of animal diseases, identifying emerging diseases, developing new diagnostic methods and training diagnosticians, and veterinarians. Recently, UDL has merged with newly formed Institute of Microbiology, UVAS. UDL serves the interests of the livestock and poultry industry, animal research scientists and educators, and companion animal owners.
In 2009, UDL became the Pakistan's first ISO 17025:2017 accredited veterinary disease diagnostic lab that provides ISO-certified diagnostic tests for the identification and monitoring emerging diseases. UDL faculty also works on developing new diagnostic methods. The lab obtained its accreditation from Pakistan National Accreditation Council (PNAC), which has mutual recognition arrangements (MRAs) with International Laboratory Accreditation Cooperation (ILAC) and Asia Pacific Laboratory Accreditation Cooperation (APLAC). It is a milestone in the history of UVAS and livestock sector of Pakistan that a veterinary disease diagnostic laboratory's results are now acceptable worldwide. The lab has also implemented a computer based "Diagnostic Information Management System (DIMS)" to ensure the good lab practices (GLP) and confidentiality of the client results. UDL provides quality diagnostic services for all animal species, including necropsy of birds, bacteriology, clinical chemistry, serology, virology, parasitology, molecular diagnostics, and toxicology.
Staff Position
COORDINATOR
PROFESSORs
ASSOCIATE PROFESSOR
ASSISTANT PROFESSOR
- Dr Muhammad Abubakar Shabbir
Publications:
Construction, molecular characterization, and safety assessment of purB mutant of Salmonella Gallinarum” Masham Mukhtar, Aamir Ghafoor, Michael McClelland, Fareeha Akhtar, Muhammad Adil Rasheed. Published in Frontiers in Microbiology 2024 |
Utilization of Fermentatively Produced Antimicrobial Peptide-Silver Nanoconjugates against Selected Bacterial Pathogens” Muhammad Usman Ahmad1†*, Kaynat William2†, Sikander Ali2 , Fareeha Akhtar3 , Saba Sana2 and Sundas Sharif published in International journal of Agriculture and Biology, 2024 |
Effect of storage temperature on the microbiological quality of fish meat from two different managemental systems. Ridda Haroon, Fareeha Akhtar, Ali Ahmad Sheikh. Accepted in Pakistan journal of zoology. 2020 |
Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat
Aftab Ahmad, Fareeha Akhtar, Muhammad Ilyas Khan, Hameed Ur Rehman, Syeda Alia Gerdazi, Uzma Ghani, Muhammad Hanzalah Khan, Uzma Ayaz, Hira Mushtaq, Nausheen Abdul Malik, Nasir Mahmood, Matiullah,Wahid Shah, Malik Nawaz Shuja. 2019. Int. J. Biosci. 14(4), 305-316, |
Study of the current resistance pattern to quinolones and other antimicrobials by multidrug resistant Escherichia coli recovered from retail chicken meat. F. Yasin, A. A. Sheikh, M. Rabbani, A. Aslam, M. I. Rasheed and R. Tanvir. The Journal of Animal & Plant Sciences, 29(6): 1580-1586. |
Immune modulatory and anti-oxidative effect of selenium against pulmonary tuberculosis. Muhammad Imran Hussain, Waqas Ahmed, Muhammad Nasir, Muhammad Hassan Mushtaq, Ali Ahmad Sheikh, Arfat Yousaf Shaheen, Shahbaz Ali and Sannaullah Iqbal (2019). Pak. J. Pharm. Sci., Vol.32, No.2(Suppl), 779-784. |
Immune boosting role of vitamin E against pulmonary tuberculosis. Muhammad Imran Hussain, Waqas Ahmed, Muhammad Nasir, Muhammad Hassan Mushtaq, Ali Ahmad Sheikh, Arfat Yousaf Shaheen and Ashraf Mahmood (2019). Pak. J. Pharm. Sci., Vol.32, No.1(Suppl), 269-276 |
Effect of carboxylated poly L-Lysine as a cryoprotectant on post-thaw quality and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull semen. Tariq, A., Ahmad, M., Iqbal, S., Riaz, M. I., Tahir, M. Z., Ghafoor, A., & Riaz, A. (2019). Theriogenology. |
Saleem N, Nawaz M, Ghafoor A, Javeed A, Mustafa A, Rizwan M. 2018. Phenotypic and Molecular Analysis of Antibiotic Resistance in Lactobacilli of Poultry Origin from Lahore, Pakistan. Pakistan Veterinary Journal. DOI: 10.29261/pakvetj/2018.084. |
Ali M, Yaqub T, Mukhtar N, Imran M, Ghafoor A, Shahid MF, Yaqub S, Smith G, Su YC, Naeem M. 2018. Prevalence and Phylogenentics of H9N2 in Backyard and Commercial Poultry in Pakistan. Avian Diseases. 62: 1-9. |
Jibreel M, Ahmad A, Rabbani M, Mushtaq H, Ghafoor A, Shabbir MZ, Saleem MH, Saleemi MK, Avais M, Muhammad J, Rehman H. 2018. Evaluation of Drinking Water Quality at Zoo and Various Public Places in District Lahore, Pakistan. Journal of Animal and Plant Sciences. 28(5): 1314-1320. |
Nasim F, Javeed A, Ashraf M, Ghafoor A. 2018. Evaluation of immunomodulatory activity of tenoxicam in mice. Tropical Journal of Pharmaceutical Research. 17(9):1811-6. |
Ali M, Yaqub T, Mukhtar N, Imran M, Ghafoor A, Shahid M, et al. 2019. Avian Influenza A (H9N2) Virus in Poultry Worker, Pakistan, 2015. Emerg Infect Dis. 25(1):136-139. Doi.org/10.3201/eid2501.180618. |
Safdar I, Sheikh AA, Akhtar F, Muhammad J , Hussain T, Gohar M, Kanwal A and Tabassum A. 2018. Optimization of multiplex PCR for detection of gram positive foodborne pathogens from retail quail meat. Pak. J. Agri. Sci. 55(4), 955-958. |
Ahmad A, Akhtar F,Khan IM, Rehman UH, Gerdazi AS, Ghani U, Khan MH, Ayaz U , Mushtaq H, Malik AN , Mahmood N, Matiullah , Shah W, Shuja NM. 2019. Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat. International Journal of Biosciences. 14(4): 305-316. |
Tanvir R, Sheikh AA, Javeed A. 2018. Endophytic Actinomycetes in the Biosynthesis of Bioactive Metabolites: Chemical Diversity and the Role of Medicinal Plants. In Studies in Natural Products Chemistry. Elsevier. 60: 399-424. |
Nawaz F, Tanvir R, Nawaz M, Javeed A, Sajid I. 2018. The effects of polar and non polar compounds from endophytic actinomycetes in Ocmium tenuiflorum (Tulsi) and Azadirachta indica (Neem) on veterinary and human pathogens. Proceedings of 4th International Electronic Conference on Medicinal Chemistry ECMC-4. Doi:10.3390/ecmc-4-05567 |
Laboratories Sections
- Bacteriology/Mycology
- Molecular Biology
- Serology
- Parasitology
Diagnostic Services
- UDL offers a wide range of testing at affordable prices (see list below).
- Scope of testing
Scope of testing
The latest tests range offered by UDL include:
TEST OFFERED AT UDL |
Rates (Rs.) |
Sample required |
POULTRY |
Postmortem for poultry (For one shed) |
500 |
Dead or Live infected birds |
ELISA for detection of antibodies against Infectious bronchitis |
800 |
Serum |
ELISA for detection of antibodies against IBD (Gumboro) virus |
800 |
Serum |
ELISA for detection of Antibodies against Mycoplasma gallisepticum |
800 |
Serum |
ELISA for detection of Antibodies against Mycoplasma synoviae |
800 |
Serum |
HI Test for antibodies against New Castle disease (ND) or Avian Influenza (AI) or Egg Drop Syndrome (EDS) viruses, (per sample) |
125 |
Serum |
Culture and sensitivity Test |
1500 |
Blood/Culture swabs( in transport media or fresh tissue)/secretions/organs |
Total viable count (water) |
1500 |
Water in sterile disposable container/Syringe |
Total viable count (feed, feed ingredients, Hatchery fluff, etc.) |
2000 |
Feed, feed ingredients, Hatchery fluff, etc (in sterile container) |
Coliform count (water) |
2000 |
Water in sterile disposable container/Syringe |
Coliform count (feed, feed ingredients, Hatchery fluff, etc.) |
2500 |
Feed, feed ingredients, Hatchery fluff, etc in sterile container) |
Isolation and identification of Salmonella (Species) |
3500 |
Cloacal swabs, tissue (liver, spleen), |
Isolation and identification of E. coli (Species) |
3000 |
Cloacal swabs, tissue (liver, spleen, fibrinous material). |
Isolation and identification of Staphylococcus (Species) |
3000 |
Pus/swabs/Milk/ tissue/Food |
Isolation and identification of Pasteurella multocida |
3000 |
(nasal/lung swabs) from sick animals and tissue samples (lung, liver, spleen, lymph nodes) from necropsy |
Isolation and identification of clostridium spp. |
5000 |
Suspected sample like feces/Intestinal contents |
Isolation and identification of bacteria from food/ commercial product |
4000 |
Food/Commercial product |
Plate exposure for bacteria (Environmental testing) / swab testing (one plate) |
2000 |
Plate exposure by lab personnel / swab testing/
Environmental swab |
Plate exposure for Yeast and Mold Count |
3000 |
Plate exposure by lab personnel / swab testing/
Environmental swab |
Antibacterial activity of disinfectant (single bacteria) |
30000 |
Disinfectant |
Antiviral activity of disinfectant (single virus) |
40000 |
Disinfectant |
Isolation and identification of Avian Influenza (AI) & New Castle disease (ND) Virus |
7000 |
Tracheal/cloacal swabs, tissue (Lungs, liver, spleen),EDTA blood |
PCR for diagnosis of Avian Influenza (H9N2) virus |
7000 |
Tracheal/cloacal swabs, tissue (Lungs, liver, spleen), EDTA blood |
PCR for diagnosis of Newcastle Disease virus |
7000 |
Tracheal/cloacal swabs, tissue (liver, spleen), EDTA blood |
PCR for diagnosis of Mycoplasma gallisepticum |
6000 |
Tracheal/cloacal swabs, tissue (Lungs, liver, spleen), EDTA blood |
PCR for diagnosis of Salmonella spp |
6000 |
Cloacal swabs |
PCR for diagnosis of Infectious Bronchitis Virus |
7000 |
Tracheal and cloacal swabs, tissue (Lungs, liver, spleen), EDTA blood |
PCR for Adenoviruses |
7000 |
Tracheal/cloacal swabs, tissue (liver, spleen), EDTA blood |
PCR for Avian collibacillosis |
5000 |
Tracheal/cloacal swabs, tissue (liver, spleen), EDTA blood |
BOVINES |
ELISA for detection of Antibodies against Foot & Mouth Disease Virus (FMDV), (Specific for Cattle & Buffalo) |
1500 |
Serum (2–3 ml clotted blood) |
ELISA for detection of Antibodies against Infection bovine Rhinotracheitis (IBR) |
1500 |
Serum (2–3 ml clotted blood) |
ELISA for detection of Antibodies against Brucella abortus |
1500 |
Serum (2–3 ml clotted blood) |
ELISA for detection of Antibodies against Johne’s disease |
1500 |
Serum (2–3 ml clotted blood) |
ELISA for detection of Antibodies against Bovine Tuberculosis (TB) |
1500 |
Serum (2–3 ml clotted blood),EDTA/Heparin vacutainer |
ELISA for detection of Bovine viral Diarrhea Virus (BVDV) |
1500 |
Serum (2–3 ml clotted blood) |
ELISA for detection of Bovine leucosis |
1500 |
Serum (2–3 ml clotted blood) |
Rose Bengal Plate test for brucellosis |
600 |
Serum (2–3 ml clotted blood) |
Tuberculin Test (Local antigen) for detection of tuberculosis |
1000 |
Live animal – intradermal test (no lab sample) |
PCR for diagnosis of Brucella abortus |
6000 |
placenta, fetal tissues |
PCR for diagnosis of Babesia bovis |
6000 |
EDTA blood |
PCR for diagnosis of Theileria annulata |
6000 |
EDTA blood |
PCR for diagnosis of Anaplasma spp |
6000 |
EDTA blood |
PCR for diagnosis of Listeria monocytogenes |
6000 |
EDTA blood /CSF/ swabs / affected tissue like placenta in sterile container/Milk/ Food |
PCR for diagnosis of Pasteurella multocida |
6000 |
Nasal swabs or respiratory lavages, or lung tissue, lymph nodes, milk. |
PCR for diagnosis of Bovine Viral diarrhea virus (BVDV) |
7000 |
EDTA whole blood, serum, milk (bulk tank or individual), and fresh ear notches (tissue) |
PCR for diagnosis of Lumpy Skin Disease Virus (LSDV) |
7000 |
EDTA blood / swabs / affected tissue in sterile container |
PCR for diagnosis of Mycobacterium tuberculosis |
6000 |
Swabs / affected tissue in sterile container |
PCR for diagnosis of Mycobacterium bovis |
6000 |
Swabs / affected tissue in sterile container |
PCR for diagnosis of Campylobacter jejuni |
6000 |
swabs / affected tissue in sterile container/Food/ semen |
PCR for diagnosis of Bovine ephemeral fever |
7000 |
EDTA blood / swabs |
PCR for diagnosis of Leptospira spp |
6000 |
Freshly collected urine sample. Transport immediately to lab |
PCR for Mycoplasma spp |
6000 |
Tracheal swabs / affected tissue like Trachea/ lungs in sterile container |
PCR for Staphylococcus aureus |
6000 |
Suspected sample/ swabs/Pus /Milk/ affected tissue in sterile container |
PCR for Streptococcus agalactiae |
6000 |
Suspected sample/ swabs/Pus /Milk/ affected tissue in sterile container |
EQUINES |
Mallein test (Local antigen) for detection of Glander |
1000 |
Live animal – intradermal test (no lab sample) |
PCR for diagnosis of Burkholderia mallei (Glander) |
7000 |
Nasal swabs / Pus/affected tissue in sterile container |
DOGS AND CATS |
HA test for Canine Parvovirus (CPV) per sample |
1000 |
Appropriate clinical sample (Stool sample/swab), rectal swab/Tissue sample |
Strip test for Canine Parvovirus (CPV) in dog |
2500 |
Appropriate clinical sample (Stool sample/swab), rectal swab/Tissue sample |
ELISA for detection of Antibodies against Rabies virus (For export documentation) |
20000 |
Serum (2–3 ml clotted blood) |
MISCELLANEOUS TESTS (Per Sample) |
Blood test for parasite (Haemoparasites) |
500 |
2-3 ml blood in EDTA containing vacutainer (Lavender top)/ Thin blood smear from peripheral blood |
Complete blood count (Haematological Analyzer) 3 part machine |
700 |
2-3 ml blood in EDTA containing vacutainer (Lavender top) |
Complete blood count (Haematological Analyzer) 4 part machine |
800 |
2-3 ml blood in EDTA containing vacutainer (Lavender top) |
White side / surf test for early detection of mastitis per sample |
500 |
Fresh milk sample (10 ml) |
Fecal examination for parasites ova detection |
500 |
Fresh feces (10–20 g) |
Fecal examination for parasites Egg per gram (EPG) |
2000 |
Fresh feces (10–20 g) |
Fecal examination for parasites (Floatation technique) |
1000 |
Fresh feces (10–20 g) |
Fecal examination for parasites (Sedimentation technique) |
1000 |
Fresh feces (10–20 g) |
Skin scrapping for detection of ectoparasites |
500 |
Deep skin scraping in sterile container |
Skin scrapping for detection of fungus |
2500 |
Deep skin scraping in sterile container, hair skin scraping ( paper envelop) |
pH of water |
1000 |
Water |
Serum Electrolytes |
Total serum / plasma Sodium |
400 |
Serum/Plasma |
Total serum / plasma Potassium |
400 |
Serum/Plasma |
Total serum / plasma Calcium |
400 |
Serum/Plasma |
Total serum / plasma Chloride |
400 |
Serum/Plasma |
Total serum / plasma protein |
400 |
Serum/Plasma |
Total serum / plasma Phosphorus |
400 |
Serum/Plasma |
Liver Function Test (LFT) |
ALP |
1500 |
Serum/Plasma |
ALT |
Serum/Plasma |
AST |
Serum/Plasma |
Total Bilirubin |
Serum/Plasma |
Renal Function Test |
Serum / plasma Creatinine |
1200 |
Serum/Plasma |
Blood Urea Nitrogen |
Serum/Plasma |
- Cost of additional services i.e. preparation of any product, visit of farm (TA/DA, Transportation charges, etc), etc., will be charged separately as per requirement.
- Lab animals are also available on cost basis.
Scope of ISO 17025 testing
|
Sr. #
|
Materials/Products tested
|
Types of test/Properties measured
|
Range of measurement
|
Minimum detection limit
|
Uncertainty of Measurement
|
Standard
|
| |
Serum
|
ELISA for detection of antibodies against IBD
|
0.25-9.0
(CPC value)
|
0.15
|
0.05
|
Kit Method (Synbiotic, USA 2025)
|
| |
Serum
|
ELISA for detection of antibodies against MG
|
0.25-9.0
(CPC value)
|
0.15
|
0.05
|
Kit Method (Bio Stone, USA 2025)
|
| |
Serum
|
ELISA for detection of antibodies against MS
|
0.25-9.0
(CPC value)
|
0.15
|
0.05
|
Kit Method (Bio Stone, USA 2025)
|
| |
Serum
|
Haemagglutination Inhibition Test for Avian Influenza (AI) H5
|
3.0-1024
(HI Titre value)
|
2.0
|
0.24
|
WOAH (Manual updated 2024)
|
| |
Serum
|
Haemagglutination Inhibition Test for Avian Influenza (AI) H7
|
3.0-1024
(HI Titre value)
|
2.0
|
0.24
|
WOAH (Manual updated 2024)
|
| |
Serum
|
Haemagglutination Inhibition Test for Avian Influenza (AI) H9
|
3.0-1024
(HI Titre value)
|
2.0
|
0.24
|
WOAH (Manual updated 2024)
|
| |
Serum
|
ELISA for detection of antibodies against Rabies
|
0.25-9.0
(CPC value)
|
0.15
|
0.05
|
Kit Method (Bio-RAD, USA 2025)
|
| |
Serum
|
ELISA for Foot & Mouth Disease (FMD)
|
0.25-9.0
(CPC value)
|
0.15
|
0.05
|
Kit Method (Prionics, USA 2025)
|
| |
Serum
|
ELISA for detection of antibodies against Brucella
|
0.25-9.0
(CPC value)
|
0.15
|
0.05
|
Kit Method (IDEXX, USA 2025)
|
| |
Serum
|
Rose Bengal Test (RBT)
|
Qualitative test
|
Not applicable
|
Not applicable
|
WOAH (Manual updated 2024)
|
| |
Water
|
pH of water
|
3:00-10:00
|
2:00
|
0.156
|
AOAC /2023, 22nd Edi, Method # 973.41
|
| |
Equine Species
|
Mallein Test
|
Qualitative test
|
Not applicable
|
Not applicable
|
WOAH (Manual updated 2024)
|
Teaching, Research & Development
UDL faculty is also involved in teaching, research & development. A number of research projects have been successfully completed and few are still in process.
|
S#
|
Title of Project
|
Source of funding
|
Amounts of funds (Rs) in Million
|
|
1.
|
Preparation of highly specific ELISA Kit on Recombinant outer membrane protein (OMP) of Pasteurella multocida B2 to detect Post-vaccination immune response
|
HEC, Pakistan
Under TDF
|
9.544
|
|
2.
|
Preparation of live genetically engineered avirulent vaccinal strains derived from local highly pathogenic isolates of P. multocida B:2, using gene deletion method and evaluation of the vaccine by inoculating in mice and calves
|
HEC, Pakistan
Under NRPU
|
6.714
|
|
3.
|
Hydrogen peroxide based product CareOx35 effect on the health of dairy cows
|
Dexon
|
0.9
|
|
4.
|
Vaccine Efficacy Testing
|
ICI
|
1.3
|
|
5.
|
Effect of Different antibiotics and steroidal Residues in Meat of Chicken and their effect on Humans in Punjab
|
National Industrial funds
|
|
|
6.
|
Epidemiology and Characterization of Bacterial Pathogens Associated with Epizootic Ulcerative Syndrome (EUS) and Motile Aeromonas Septicemia (MAS) from Pakistani Farmed Fish
|
National (PARC)
|
|
|
7.
|
Molecular Characterization of Bovine Pestivirus isolates from dairy animal of Pakistan and their use in diagnostic, biosecurity and biological development to control disease in Pakistan herds
|
National (HEC)
|
6.022
|
|
8.
|
Comparative transcriptome analysis of innate and adaptive immune cells during SARS-CoV-2 infection
|
International (Qatar)
|
5.5
|
Human Resource Development
UDL is playing a vital role in human resource development through organizing workshops, providing trainings, refresher courses and diploma courses for UVAS staff, students, farmers, lab and field professionals and personnel from sister institutions of the country.
International Linkages
|
Sr. No.
|
University / College
|
Country
|
Nature of Cooperation
|
|
1
|
Division of Lab Animal Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA
|
USA
|
Faculty development in Lab Animal Facility Management
|
|
2
|
Department of Pathobiology, The University of Connecticut, Connecticut
|
USA
|
Collaborative Research & Faculty Development
|
|
3
|
Institute of Animal Health, Compton
|
UK
|
- do -
|
|
4
|
Animal Diagnostic Laboratory, PennState University
|
USA
|
- do -
|
|
5
|
Atlantic Veterinary College, University of Prince Edward Island
|
Canada
|
Faculty Development
|
|
6
|
Agriculture & Agri Food Cooperation (AAFC)
|
Canada
|
- do -
|
|
7
|
University of Qatar
|
Qatar
|
Collaborative research
|
|
8
|
Vaccine and Infectious Disease Organization- International Vaccine Center (VIDO-InterVac)
|
Canada
|
Collaborative research
|
Clients
- K & Ns Poultry Farms, Raiwind, Lahore
- Supreme Farms, Lahore-Sheikhupura Road
- The Livestock Farm, Rakh Dera Chahl, Lahore
- RVFC (Mona depot, Abbotabad, Sahiwal etc.)
- The Governor House, Lahore
- The Zoo, Lahore
- The Livestock Farms of the Punjab
- The Clinics of UVAS, Lahore.
- The Pets Clinics, Lahore
- The Camel Owners, Lahore
- The Kennel Club, Lahore
- National Feed Pvt. Ltd, Raiwind, Lahore
- The Race Course Club, Lahore.
- The Rangers Lahore
- The Police Line, Lahore
- Rana Nisar Stud Farm, Phool Nagar, Pattoki
- Munir Poultry Farm, Gujranwala
- Idara-e-Kissan
- The Crystal Feed Industry, Multan
- Noor Stud Farm, Sharif City, Raiwind Road
- Other Poultry Farmers of Punjab province
- Private Veterinary Clinics
Location
University Diagnostic Lab (UDL), University of Veterinary and Animal Sciences – UVAS, Syed Abdul Qadir Jillani (Out Fall) Road, 54000 Lahore – Pakistan
Regular Business Hours
Monday – Friday
8: 00a.m. - 4:00 p.m.
Parking
Temporary parking for UDL clients on four wheels is available in front of UDL. For motor bikes, in the parking stand on main outfall road. |
