Dr. Muhammad Wasim                               

M.Sc. (BZU), M.Phil (QAU), PhD (AUSTRIA)
Associate Professor

HEC Approved Supervisor

Telephone: +92-42-99211449-450 & +92-42-99211374 Ext: 302
Mobile: +92-300-7316331
Email: muhammad.wasim@uvas.edu.pk

Biography

Dr. Muhammad Wasim earned his PhD degree in Molecular Biology/Molecular Oncology from Medical University of Innsbruck, AUSTRIA. During his PhD, Dr. Wasim has got opportunity to present his research work at many International forums in Canada, Luxemburg as well as in Austria. Dr. Wasim has vast experience of teaching Biology/Molecular Biology at college and University level. He has supervised/supervising many M.Phil and PhD students.  Dr. Wasim has published a number of research articles in peer-reviewed International journals. He has completed three research projects in Austria and running an HEC funded research project currently. His research interests are the study of genes involved in cancers (Oncogenes and tumor suppressor genes) as well as other human and animal disorders and their expression analysis using high throughput techniques.

Areas of Interest

Study of gene function and expression involved in cancers (animal & Human) and other disorders

Publications


  1. “Quantitative PCR” In: Book “Polymerase Chain Reaction”, VDM GmbH & Co. KG, Germany pp. 123-137, 2011.
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Barbara Gandolfi, Hasan Alhaddad, Mona Abdi, Leslie H. Bach, Erica K. Creighton, Brian W. Davis, Jared E. Decker, Nicholas H. Dodman, Jennifer C. Grahn, Robert A. Grahn, Bianca Haase, Jens Haggstrom, Michael J. Hamilton Christopher R. Helps, Jennifer D. Kurushima, Hannes Lohi, Maria Longeri, Richard Malik, Kathryn M. Meurs, Michael J. Montague, James C. Mullikin, William J. Murphy, Sara M. Nilson, Niels C. Pedersen, Carlyn B. Peterson, Clare Rusbridge, Rashid Saif, Diane G. Shelton, Wesley C. Warren, Muhammad Wasim & Leslie A. Lyons 2018. Applications and efficiencies of the first cat 63K DNA array. Scientific
Reports. 8:7024 DOI:10.1038/s41598-018-25438-0.

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Muhammad Javed Iqbal, Muhammad Wasim, Umer Rashid, Nadia Zeeshan, Rizwan Ali, Sawera Nayyab, Sahrish Habib, Bushra Manzoor, Nouman Zahid 2018. Mutational screening of GABRG2 gene in Pakistani population of Punjab with generalized tonic clonic seizures and children with childhood absence epilepsy. Journal of the Chinese Medical Association. https://doi.org/10.1016/j.jcma.2018.05.001
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Sabah Mansoor, Muhammad Tayyab, Amna Jawad, Bushra Munir, Sehrish Firyal, Ali Raza Awan, Naeem Rashid and Muhammad Wasim 2018.Refolding of Misfolded Inclusion Bodies of Recombinant a-Amylase: Characterization of Cobalt Activated Thermostable a-Amylase from Geobacillus SBS-4S. Pakistan J. Zool., vol. 50(3), pp 1147-1155, 2018.

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Firyal, S., A.R. Awan, H. Sattar, H.K.R. Ullah, M. Tayyab, M. Wasim, S. Saeed, R. Naseer, M.O. Umer and I. Rashid, 2018. Identification of polymorphism in bovine tumor necrosis factor alpha and toll-like receptor 4 genes and its association with mastitis in Sahiwal cows. Int. J. Agric. Biol., 20: 750-752
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Shakeela Daud, Nisar Ahmed, Sara Naudhani, Muhammad Younus, Saba Manzoor, Sehrish Firyal, Tahir, Yaqub, Abu Saeed Hashmi, Riaz ul Amin, Muhammad Wasim and Sajid Umer 2018. In-Silico Analysis of Gene ALS2 Genetic Variants Identified in the Affected Horses and Humans With Motor Neuron Disease. Journal of Equine Veterinary Science, 62: 60-65
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Naveed Hussain, Abu Saeed Hashmi, Muhammad Wasim, Tauqeer Akhtar,  Shagufta Saeed and Toheed Ahmad 2018. Synergistic potential of Zingiber officinale and Curcuma longa to ameliorate diabetic-dyslipidemia. Pakistan Journal of Pharmaceutical Sciences, Vol.31, No.2, March 2018, pp.491-498

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Rashid Saif, Ali Raza Awan, Muhammad Tayyab, Masroor Ellahi Babar, Asim Khalid Mahmood, Zia Ullah, Saeeda Zia, Muhammad Wasim 2017. Expression Profiling of Hspb1 and Tp53 Genes through RT-qPCR in Diff erent Cancer Types of Canis familiaris. Iranian Journal of Biotechnology. 2017 July;15(3):e1505

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Sehrish Firyal, Sidra Mukhtar, Ali Raza Awan, M. Tayyab, M. Wasim, Shagufta Saeed and Ghulam Muhammad 2017. Polymorphism Analysis of Exon 2, 5 and 10 of Bovine Lactoferrin Gene and its Association with Mastitis in Sahiwal Cows. Pakistan Veterinary Journal, 37(4): 480-481.

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Saba Manzoor, Ali Raza Awan, Abdul Wajid, Sehrish Firyal, Muhammad Mansha, Muhammad Tayyab, Asim Khalid Mehmood, Abu Saeed Hashmi and Muhammad Wasim 2017. c-Myc has Altered Expression in Canine and Feline Tumors. Pakistan Journal of Zoology. 49(6):2147-2152
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Furqan Sabir, Muhammad Tayyab, Bushra Munir, Abu Saeed Hashmi, Ali Raza Awan, Naeem Rashid, Muhammad Wasim and Sehrish Firyal 2017. Characterization of Recombinant Thermostable Phytase from Thermotoga naphthophila: A Step for the Fulfilment of Domestic Requirement of Phytase in Pakistan. Pakistan Journal of Zoology. 49(6):1945-1951

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Rashid Saif , Ali Raza Awan, Muhammad Tayyab, Masroor Ellahi Babar, Asim Khalid Mahmood, Zia Ullah, Saeeda Zia, Muhammad Wasim 2017. Expression Profiling of Hspb1 and Tp53 Genes through RT-qPCR in Different Cancer Types of Canis familiaris. Iranian Journal of Biotechnology. 15(3): e1505

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Javeria, Masroor Ellahi Babar, Akhtar Ali, Asif Nadeem, Abdul Wajid, Sajjad Ali Shah, Sadaf Rashid, Muhammad Wasim and Muhammad Abdullah 2017. Association between the COMT Gene and Obsessive Compulsive Disorder: A Case-Control Study. Pakistan Journal of Zoology. 49(5):1921-1924

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A. Wajid, M. Wasim, A. Batool, H. Sadia, T. Bibi, A. Basharat, S. Manzoor, T. Yaqub, M. Tayyab and S. F. Rehmani 2017. COMPLETE GENOMIC ANALYSIS OF NEWCASTLE DISEASE VIRUS OF A RECENT PANZOOTIC ISOLATED FROM VACCINATED POULTRY FLOCK IN 2014 IN PAKISTAN. Journal of Animal and Plant Sciences. 27(5): 1504-1514

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Anum Uzair, Nouman Rasool, Muhammad Wasim 2017. Evaluation of different methods for DNA extraction from human burnt bones and the generation of genetic profiles for identification. Medicine, Science and the Law. 57(4):159-166
http://journals.sagepub.com/eprint/gp7yM8rREqZy89ZIsIZN/full
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Javeria, Masroor Ellahi Babar, Tanveer Hussain, Rashid Saif, Sadaf Rashid, Hanan Sarfraz, Abbas Ali Shah, Muhammad Wasim and Muhammad Abdullah 2017. Genetic Contribution of BDNF Gene in Obsessive Compulsive Disorder in Pakistani Punjabi Population. Pakistan Journal of Zoology. 49(4):1523-1526
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J. Muhammad, M. Rabbani, K. Muhammad, M. Wasim, K. Dewan, A. A. Anjum, A. A. Sheikh, A. Ahmad, F. Akhtar, M. Nisar, B. M. Jayarao and G. S Kirimanjeswara 2017. LIPOPOLYSACHARIDE GENE BASED DEVELOPMENT AND OPTIMIZATION OF DIAGNOSTICS FOR FRANCISELLA TULARENSIS. Journal of Animal and Plant Sciences. 27(4): 1161-1166

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J. Muhammad, M. Rabbani, K. Muhammad, M. Wasim, A. Ahmad, A. A. Sheikh, F. Akhtar, A. Rasool, I. Khattak, T. Bashir, Z. U. Islam, M. Rasheed 2017. PHYSICOCHEMICAL FACTORS AFFECTING PERSISTENCE OF FRANCISELLA TULARENSIS IN SOIL. Journal of Animal and Plant Sciences. 27(3): 1047-1050

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Rashid Saif, Ali Raza Awan, Leslie A. Lyons, Barbara Gandolfi, Muhammad Wasim 2017. Exploration of novel somatic Hspb1 mutations in widespread neoplasms of Canis familiaris. International Journal of Biosciences. 10(5): 265-272
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Abdul Wajid, Kiril M. Dimitrov, Muhammad Wasim, Shafqat Fatima Rehmani, Asma Basharat, Tasra Bibi, Saima Arif, Tahir Yaqub, Muhammad Tayyab, Mustafa Ababneh, Poonam Sharma, Patti J. Miller, Claudio L. Afonso 2017. Repeated isolation of virulent Newcastle disease viruses in poultry and captive non-poultry avian species in Pakistan from 2011 to 2016. Preventive Veterinary Medicine. 142: 1-6 https://doi.org/10.1016/j.prevetmed.2017.04.010
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Wajid A, Basharat A, Khan TA, Wasim M, Rehmani SF 2017. Complete Genome Sequence of a Velogenic Newcastle Disease Virus Strain Isolated from a Clinically Healthy Exotic Parakeet (Melopsittacus undulatus) in Pakistan. Genome Announc. 5(6): e01581-16

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Ali Raza Awan, Sehrish Firyal, Muhammad Tayyab, Lala Rukh, M. Zia ul Haq, Shagufta Saeed and Muhammad Wasim 2017. Molecular Classification of Pakistani Wild Rose-Ringed Parakeet through Novel Cytochrome B Gene Polymorphism. Pakistan Journal of Zoology. 49(1):267-271

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Shakeela Daud, Sara Naudhani, NisarAhmed, Tahir Yaqub, Abu Saeed Hashmi, Ali Raza Awan, Abdul Wali, Muhammad Luqman, Muhammad Wasim 2017. Mutational analysis of the equine Cu/Zn superoxide dismutase (SOD1) and amyotrophic lateral sclerosis (ALS2) in horses of Pakistani origin: No evidence found for motor neuron disease. International Journal of Biosciences. 10(3): 157-162

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Saif, R.; Awan, A. R.; Lyons, L.; Gandolfi, B.; Tayyab, M.; Ellahi Babar, M. and Wasim, M. 2016. Mammary tumor associated Hspb1 mutation and screening of eight cat populations of the world. Iranian Journal of Veterinary Research. 17(4): 231-236
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Rashid Saif , Ali Raza Awan, Leslie Lyons, Barbara Gandolfi, Muhammad Tayyab , Masroor Ellahi Babar, Asim Khalid Mehmood , Zia Ullah , Muhammad Wasim 2016. Hspb1 and Tp53 Mutation and Expression Analysis in Cat Mammary Tumors. Iranian Journal of Biotechnology. 14(3): e1480

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Haleema Sadia, Saba Manzoor, Abdul Wajid, Muhammad Tayyab, Sehrish Firyal, Abu Saeed Hashmi, Zia Ullah Mughal, Asim Khalid Mehmood, Tahir Yaqub, Ali Raza Awan and Muhammad Wasim 2016. Feline Mammary Tumors Show Altered COX-2 and BRCA1 Expression. Pakistan Journal of Zoology. 48:1705-1714

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Tanveer Hussain, Masroor Ellahi Babar, Sunday O. Peters, Abdul Wajid, Akhtar Ali, Amber Azam, Zahoor Ahmad, Muhammad Wasim, Ahmad Ali, Kadir Kizilkaya, Marcos De Donato and Ikhide G. Imumorin 2016. Microsatellite Markers Based Genetic Evaluation of Pakistani Cattle Breeds. Pakistan Journal of Zoology. 48:1633-1641

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Saif R, Khan E, Azhar A, Choudhary S, Hussain T, Babar ME, Awan AR, Tayyab M, Zia S, Wasim M. 2016. Insight of Tp53 Mutations and their effect on Protein in Different Feline and Canine Neoplasms. Advancement in Life Sciences. 3(2): 42-50.

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Saeeda Kalsoom, Khushnooda Ramzan, Honainah Ishaq Tahir, Ali Raza Awan, Aftab Ahmed Anjum and Muhammad Wasim 2016. In Silico Analysis of Missense Substitutions in RB1 Gene and their Effect on Metabolic Pathways. Pakistan Journal of Zoology. 48: 639-644
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Naeem Aslam, Asif  Nadeem, Masroor Ellahi Babar, Muhammad Tariq Pervez, Maryam Javed, Muhammad Aslam, Muhammad Wasim and Wasim Shehzad 2016.  RPDB: A Relational Databank of Protein Structures. Pakistan Journal of Agricultural Sciences. 53(1): ISSN (Print) 0552-9034, ISSN (Online) 2076-0906
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Muhammad Mansha, Maryam Saleem, Muhammad Wasim, Muhammad Tariq 2016.  Prevalence of Known Risk Factors in Women Diagnosed with Breast Cancer at Inmol Hospital, Lahore, Punjab. Asian Pacific Journal of Cancer Prevention. 17 (2): 563-568
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Naeem Aslam, Asif  Nadeem, Masroor Ellahi Babar, Muhammad Tariq Pervez, Muhammad Aslam, Nasir Naveed, Tanveer Hussain, Wasim Shehzad, Muhammad Wasim, Zhang Bao, MaryamJaved 2016. The accuracy of protein structure alignment servers. Electronic Journal of Biotechnology. 20: 9-13
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H. Sadia, S. Manzoor, A. Wajid, M. Tayyab, S. Firyal, A. S. Hashmi, T. Yaqub, Z. U. Mughal, A. K. Mehmood, A. R. Awan, K. Mahmood, M. Y. Khan and M. Wasim 2016. COX-2 and BRCA1 have altered expression profile in different canine tumors. Journal of Animal and Plant Sciences. 26(1): 103-115
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Shakeela Daud, Naseebullah Kakar, Ingrid Goebel, Abu Saeed Hashmi, Tahir Yaqub, Gudrun Nürnberg, Peter Nürnberg, Deborah J. Morris-Rosendahl, Muhammad Wasim, Alexander E. Volk, Christian Kubisch, Jamil Ahmad & Guntram Borck 2016. Identification of two novel ALS2 mutations in infantile-onset ascending hereditary spastic paraplegia. Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration. 17:3-4, 260-265
DOI: 10.3109/21678421.2015.1125501

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Abdul Wajid, Muhammad Wasim, Shafqat F. Rehmani, Tasra Bibi, Nazir Ahmed, Claudio L. Afonso 2015. Complete Genome Sequence of a Recent Panzootic Virulent Newcastle Disease Virus from Pakistan. Genome Announcement. 3(3): e00658-15. doi:  10.1128/genomeA.00658-15
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Asima Saman, Abdul Wajid, Muhammad Wasim, Aysha Sara, Muhammad Salman and Zia Uddin 2016. Allele and haplotype diversity of 17 Y-STRs loci in the northern region Pashtun (Pathans) population of Pakistan. Australian Journal of Forensic Sciences. 48:3,
273-278 DOI: 10.1080/00450618.2015.1112425

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Muhammad Tayyab, Naeem Rashid, Clement Angkawidjaja, Shigenori Kanaya, Muhammad Wasim, Ali Raza Awan, Sehrish Firyal, Tahir Yaqub and Masood Ahmed Siddiqui 2015.  Hydrophobic interactions induced activation of a thermo-alkalophilic lipase from Geobacillus SBS-4S by molecular dynamics simulations.  Journal of Chemical Society of Pakistan. 37:1030-1032

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Saeeda Kalsoom, Muhammad Wasim, Sibtain Afzal, Muhammad Saqib Shahzad, Shaiqa Ramzan, Ali Raza Awan, Aftab Ahmed Anjum, Khushnooda Ramzan 2015. Alterations in the RB1 gene in Pakistani patients with retinoblastoma using direct sequencing analysis. Molecular Vision. 21:1085-1092

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J. Muhammad, A. A. Anjum, M. Rabbani, K. Muhammad, M. Wasim, A. Ahmad, A. A. Sheikh, R. K. Khalid, A. Y. Shaheen and F. Liaqat 2015.  In Vitro efficacy of Garlic, glycyrrhiza and Neem Against Local isolates of Mycoplasma Gallisepticum. Journal of Animal and Plant Sciences. 25(1): 295-297
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Maryam Javed, Masroor Ellahi Babar, Asif Nadeem, Muhammad Wasim, Tanveer Hussain, Abu Saeed Hashmi 2014. Biological Networking of Proteins Having Impending Role in Milk Fat Content of Bovines. Life Science Journal. 11(10s):659-663

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A. Wajid, M. Wasim, T. Yaqub, S. Firyal, M. Tayyab, S. Siddique,  Tanveer Hussain 2014. Assessment of genetic diversity in Balochi and Rakhshani sheep breeds of Balochistan using microsatellite DNA markers. Journal of Animal and Plant Sciences. 24(5): 1348-1354

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Muhammad WASIM, Muhammad MANSHA, Muhammad TAYYAB, Ali Raza AWAN, Sehrish FIRYAL, Christine MANTINGER, Tahir YAQUB 2014. PLZF over-expression in T-ALL cell line CEM-C7H2-2C8 down-regulates glucocorticoid receptor (GR) and its target genes. Turkish Journal of Biology. 38: 56-959

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Muhammad MANSHA, Muhammad WASIM, Ali Raza AWAN, Asma Abdul LATIF 2014. Analysis of “Bimbam”, a novel glucocorticoid-induced BH3-only transcript in cell lines and children with acute lymphoblastic leukemia. Turkish Journal of Biology. 38: 906-915

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Sehrish Firyal,Tahir Yaqub, Aftab Ahmad Anjum, Muddasar Saeed Khan, Muhammad Mansha, Muhammad Tayyab, Muhammad Wasim and Ali Raza Awan 2014. Molecular Classification of Pakistani Rose-Ringed Parakeet using Mitochondrial ND2 Gene. International Journal of Agriculture and Biology. 16: 1209–1212

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Hira Naseer, Sehrish Firyal, Tahir Yaqub, Muhammad Tayyab, Aftab Ahmad Anjum, Muhammad Yasir Zahoor, Muhammad Wasim, Nadia Mukhtar and Ali Raza Awan 2014.  Molecular basis of tetracycline resistance in Escherichia coli isolates recovered from poultry drinking water. International Journal of Agriculture and Biology. 16: 227‒230
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Abdul Hameed Baloch, Shakeela Daud, Nafeesa Raheem, Muhammad Luqman,  Adeel Ahmad, Abdul Rehman, Saeeda Rasheed, Akhtar Ali, Naseebullah Kakar, Hafiz Khush naseeb, Muhammad Arif Awan, Masroor Ellahi Babar, Muhammad Wasim, Dost Mohammad Baloch and Jamil Ahmad 2014. Missense mutations (p.H371Y, p.D438Y) in gene CHEK2 are associated with breast cancer risk in women of Balochistan origin. Molecular Biology Reports. 41:1103–1107

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A Wajid, T Hussain,  M Wasim, ME Babar, AA Anjum, SA Shah, K Abbas 2013. The future prospective of genomic biotechnology in animal breeding: their potential for livestock production in Pakistan. Journal of Animal and Plant Sciences. 23 (4), 944-955
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Rashid Saif, Muhammad Wasim, Masroor Ellahi Babar 2012. Molecular Phylogeny of Pakistani Riverine Buffalo Based on Genetic variability of Mitochondrial Cytochrome b Gene. Molecular Biology Reports. 39(10):9707-14
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Muhammad Mansha, Muhammad Wasim, Christian Ploner, Abrar Hussain , Asma Abdul Latif, Muhammad Tariq and Anita Kofler 2012. Problems encountered in bicistronic ires-GFP expression vectors employed in functional analyses of GC-induced genes. Molecular Biology Reports. 39(12):10227-34
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Mansha M, Wasim M, Kofler A, Ploner C 2012. Expression and glucocorticoid-regulation of "Bam", a novel BH3-only transcript in acute lymphoblastic leukemia. Molecular Biology Reports. 39(5):6007-13
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Muhammad Wasim, Muhammad Mansha, Anita Kofler, Ali Raza Awan, Masroor Ellahi Babar, Reinhard Kofler 2012. Promyelocytic leukemia zinc finger protein (plzf) enhances glucocorticoid-induced apoptosis in leukemic cell line Nalm6. Pakistan Journal of Pharmaceutical Sciences. 25 (3):617-621
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Muhammad Wasim, Michela Carlet, Johannes Rainer, Muhammad Mansha, Alexander Trockenbacher, Reinhard Kofler 2010. PLZF/ZBTB16, a glucocorticoid response gene in acute lymphoblastic leukemia, interferes with glucocoticoid-induced apoptosis. J. Steroid Biochem Mol Biol. 120(4-5):218-27
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Muhammad Mansha, Michela Carlet, Christian Ploner, Georg Gruber, Muhammad Wasim, Jan Wiegers, Johannes Rainer, Stephan Geley, Reinhard Kofler 2010.  Functional analyses of Src-like adaptor (SLA), a glucocorticoid-regulated gene in acute lymphoblastic leukemia. Leukemia Research. 34(4):529-34
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Rainer J, Ploner C, Jesacher S, Ploner A, Eduardoff M, Mansha M, Wasim M, Panzer-Grümayer R, Trajanoski Z, Niederegger H, Kofler R. 2009. Glucocorticoid-regulated microRNAs and mirtrons in acute lymphoblastic leukemia. Leukemia. 23(4):746-52

 

 

  1. Wasim M. (2010) Micro- & Mini-satellte and SNP analysis, National Conference on Animal Forensics: DNA Fingerprinting, University of Veterinary & Animal Sciences, Lahore-Pakistan. September 21-22, 2010.
  2. Wasim M. (2010) Stem Cell Technology, Proceedings of Proceedings of 2nd International Symposium on Biomedical Materials: Current Research and Future Signposting 2010, Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Defence Road, Off Raiwind Road, Lahore, July 6-7, 2010.
  3. Wasim M. (2010) Mosquito as Possible Vector in Hepatitis. Hepatitis Day Seminar, University of Veterinary and Animal Sciences, Lahore, May 19, 2010. 
  4. Wasim M. (2010) In silico generation of GATEWAYTM compatible expression vector using Vector NTI software. Proceedings of I National Conference on Bioinformatics: Opening Up New Frontiers in Molecular Biology Research” Lahore, Pakistan May 14-15, 2010.
  5. Baber M. E and Wasim M. (2009) Introduction to animal stem cell therapy. Proceedings of International conference on Role of Stem Cell Technology in Animal Sciences, December 4-5, 2009.