Our mission is to protect and promote animal and human health through early detection and monitoring of animal diseases.
The University Diagnostic Lab (UDL) of the University of Veterinary & Animal Sciences, (UVAS) Lahore is a national leader in providing rapid diagnosis of animal diseases, identifying emerging diseases, developing new diagnostic methods and training diagnosticians, and veterinarians. Recently, UDL has merged with newly formed Institute of Microbiology, UVAS. UDL serves the interests of the livestock and poultry industry, animal research scientists and educators, and companion animal owners.
In 2009, UDL became the Pakistan's first ISO 17025 accredited veterinary disease diagnostic lab that provides ISO-certified diagnostic tests for the identification and monitoring emerging diseases. UDL faculty also works on developing new diagnostic methods. The lab obtained its accreditation from Pakistan National Accreditation Council (PNAC), which has mutual recognition arrangements (MRAs) with International Laboratory Accreditation Cooperation (ILAC) and Asia Pacific Laboratory Accreditation Cooperation (APLAC). It is a milestone in the history of UVAS and livestock sector of Pakistan that a veterinary disease diagnostic laboratory's results are now acceptable worldwide. The lab has also implemented a computer based "Diagnostic Information Management System (DIMS)" to ensure the good lab practices (GLP) and confidentiality of the client results. UDL provides quality diagnostic services for all animal species, including necropsy of birds, bacteriology, clinical chemistry, serology, virology, parasitology, molecular diagnostics, and toxicology.
Staff Position
COORDINATOR
PROFESSORs
ASSOCIATE PROFESSOR
ASSISTANT PROFESSOR
Publications:
Complete Genome Sequences of 16 Mycoplasma bovis Isolates from Canadian Bison and Cattle. Karen B Register, Darrell O Bayles, Hao Ma, M Claire Windeyer, Jose Perez-Casal, Ana L Bras, Muhammad Suleman, Murray Woodbury, Murray D Jelinski, David P Alt. Microbiol Resour Announc. 2020 Jun 4;9(23):e00325-20. doi: 10.1128/MRA.00325-20. |
Effect of storage temperature on the microbiological quality of fish meat from two different managemental
systems. Ridda Haroon, Fareeha Akhtar, Ali Ahmad Sheikh. Accepted in Pakistan journal of zoology. 2020 |
Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat
Aftab Ahmad, Fareeha Akhtar, Muhammad Ilyas Khan, Hameed Ur Rehman, Syeda Alia Gerdazi, Uzma Ghani, Muhammad Hanzalah Khan, Uzma Ayaz, Hira Mushtaq, Nausheen Abdul Malik, Nasir Mahmood, Matiullah,Wahid Shah, Malik Nawaz Shuja. 2019. Int. J. Biosci. 14(4), 305-316, |
Study of the current resistance pattern to quinolones and other antimicrobials by multidrug resistant Escherichia coli recovered from retail chicken meat. F. Yasin, A. A. Sheikh, M. Rabbani, A. Aslam, M. I. Rasheed and R. Tanvir. The Journal of Animal & Plant Sciences, 29(6): 1580-1586. |
Immune modulatory and anti-oxidative effect of selenium against pulmonary tuberculosis. Muhammad Imran Hussain, Waqas Ahmed, Muhammad Nasir, Muhammad Hassan Mushtaq, Ali Ahmad Sheikh, Arfat Yousaf Shaheen, Shahbaz Ali and Sannaullah Iqbal (2019). Pak. J. Pharm. Sci., Vol.32, No.2(Suppl), 779-784. |
Immune boosting role of vitamin E against pulmonary tuberculosis. Muhammad Imran Hussain, Waqas Ahmed, Muhammad Nasir, Muhammad Hassan Mushtaq, Ali Ahmad Sheikh, Arfat Yousaf Shaheen and Ashraf Mahmood (2019). Pak. J. Pharm. Sci., Vol.32, No.1(Suppl), 269-276 |
Effect of carboxylated poly L-Lysine as a cryoprotectant on post-thaw quality and in vivo fertility of Nili Ravi buffalo (Bubalus bubalis) bull semen. Tariq, A., Ahmad, M., Iqbal, S., Riaz, M. I., Tahir, M. Z., Ghafoor, A., & Riaz, A. (2019). Theriogenology. |
Saleem N, Nawaz M, Ghafoor A, Javeed A, Mustafa A, Rizwan M. 2018. Phenotypic and Molecular Analysis of Antibiotic Resistance in Lactobacilli of Poultry Origin from Lahore, Pakistan. Pakistan Veterinary Journal. DOI: 10.29261/pakvetj/2018.084. |
Ali M, Yaqub T, Mukhtar N, Imran M, Ghafoor A, Shahid MF, Yaqub S, Smith G, Su YC, Naeem M. 2018. Prevalence and Phylogenentics of H9N2 in Backyard and Commercial Poultry in Pakistan. Avian Diseases. 62: 1-9. |
Jibreel M, Ahmad A, Rabbani M, Mushtaq H, Ghafoor A, Shabbir MZ, Saleem MH, Saleemi MK, Avais M, Muhammad J, Rehman H. 2018. Evaluation of Drinking Water Quality at Zoo and Various Public Places in District Lahore, Pakistan. Journal of Animal and Plant Sciences. 28(5): 1314-1320. |
Nasim F, Javeed A, Ashraf M, Ghafoor A. 2018. Evaluation of immunomodulatory activity of tenoxicam in mice. Tropical Journal of Pharmaceutical Research. 17(9):1811-6. |
| Ali M, Yaqub T, Mukhtar N, Imran M, Ghafoor A, Shahid M, et al. 2019. Avian Influenza A (H9N2) Virus in Poultry Worker, Pakistan, 2015. Emerg Infect Dis. 25(1):136-139. Doi.org/10.3201/eid2501.180618. |
Safdar I, Sheikh AA, Akhtar F, Muhammad J , Hussain T, Gohar M, Kanwal A and Tabassum A. 2018. Optimization of multiplex PCR for detection of gram positive foodborne pathogens from retail quail meat. Pak. J. Agri. Sci. 55(4), 955-958. |
Ahmad A, Akhtar F,Khan IM, Rehman UH, Gerdazi AS, Ghani U, Khan MH, Ayaz U , Mushtaq H, Malik AN , Mahmood N, Matiullah , Shah W, Shuja NM. 2019. Comparative antibiotic resistance profile of Acinetobacter spp. isolated from fish, chicken and beef meat. International Journal of Biosciences. 14(4): 305-316. |
Tanvir R, Sheikh AA, Javeed A. 2018. Endophytic Actinomycetes in the Biosynthesis of Bioactive Metabolites: Chemical Diversity and the Role of Medicinal Plants. In Studies in Natural Products Chemistry. Elsevier. 60: 399-424. |
Nawaz F, Tanvir R, Nawaz M, Javeed A, Sajid I. 2018. The effects of polar and non polar compounds from endophytic actinomycetes in Ocmium tenuiflorum (Tulsi) and Azadirachta indica (Neem) on veterinary and human pathogens. Proceedings of 4th International Electronic Conference on Medicinal Chemistry ECMC-4. Doi:10.3390/ecmc-4-05567 |
Laboratories Sections
- Bacteriology/Mycology
- Molecular Biology
- Serology
- Parasitology
Diagnostic Services
UDL offers a wide range of testing at affordable prices (see list below).
Scope of testing
The latest tests range offered by UDL include:
TEST OFFERED AT UDL |
Rates (Rs.) |
POULTRY |
Postmortem for poultry (For one shed) |
300 |
ELISA for detection of antibodies against Infectious bronchitis |
500 |
ELISA for detection of antibodies against IBD (Gumboro) virus |
500 |
ELISA for detection of Antibodies against Mycoplasma gallisepticum |
500 |
ELISA for detection of Antibodies against Mycoplasma synoviae |
500 |
HI Test for antibodies against New Castle disease (ND) or Avian Influenza (AI) or Egg Drop Syndrome (EDS) viruses, (per sample) |
80 |
Culture and sensitivity Test |
1000 |
Total viable count (water) |
1200 |
Total viable count (feed, feed ingredients, Hatchery fluff, etc.) |
2000 |
Coliform count (water) |
1500 |
Coliform count (feed, feed ingredients, Hatchery fluff, etc.) |
2000 |
Isolation and identification of Salmonella (Species) |
3000 |
Isolation and identification of Cryonic |
3000 |
Isolation and identification of E. coli (Species) |
2500 |
Isolation and identification of Staphylococcus (Species) |
2500 |
Isolation and identification of Pasteurella multocida |
2500 |
Isolation and identification of Mycoplasma gallisepticum |
3000 |
Isolation and identification of clostridium spp |
5000 |
Isolation and identification of bacteria from food/ commercial product |
3500 |
Plate exposure for bacteria (Environmental testing) / swab testing (one plate) |
2000 |
Plate exposure for Yeast and Mold Count |
3000 |
Antibacterial activity of disinfectant (single bacteria) |
30000 |
Antiviral activity of disinfectant (single virus) |
35000 |
Isolation and identification of Avian Influenza (AI) & New Castle disease (ND) Virus |
5000 |
PCR for diagnosis of Avian Influenza (H9N2) virus |
5000 |
PCR for diagnosis of Newcastle Disease virus |
5000 |
PCR for diagnosis of Mycoplasma gallisepticum |
5000 |
PCR for diagnosis of Salmonella spp |
5000 |
PCR for diagnosis of Infectious Bronchitis Virus |
5000 |
BOVINES |
ELISA for detection of Antibodies against Foot & Mouth Disease Virus (FMDV), (Specific for Cattle & Buffalo) |
1000 |
ELISA for detection of Antibodies against Infection bovine Rhinotracheitis (IBR) |
1000 |
ELISA for detection of Antibodies against Brucella abortus |
1000 |
ELISA for detection of Antibodies against Johne’s disease |
1000 |
ELISA for detection of Antibodies against Bovine Tuberculosis (TB) |
1000 |
ELISA for detection of Bovine viral Diarrhea Virus (BVDV) |
1000 |
ELISA for detection of Bovine leucosis |
1000 |
Rose Bengal Plate test for brucellosis |
500 |
Tuberculin Test (Local antigen) for detection of tuberculosis |
500 |
PCR for diagnosis of Brucella abortus |
5000 |
PCR for diagnosis of Babesia bovis |
3000 |
PCR for diagnosis of Theileria annulata |
3000 |
PCR for diagnosis of Anaplasma spp |
3000 |
PCR for diagnosis of Listeria monocytogenes |
5000 |
PCR for diagnosis of Pasteurella multocida |
5000 |
PCR for diagnosis of Bovine Viral diarrhea virus (BVDV) |
5000 |
PCR for diagnosis of Lumpy Skin Disease Virus (LSDV) |
5000 |
PCR for diagnosis of Mycobacterium tuberculosis |
5000 |
PCR for diagnosis of Mycobacterium bovis |
5000 |
PCR for diagnosis of Campylobacter jejuni |
5000 |
PCR for diagnosis of Bovine ephemeral fever |
5000 |
PCR for diagnosis of Leptospira spp |
5000 |
EQUINES |
Mallein test (Local antigen) for detection of Glander |
1000 |
PCR for diagnosis of Burkholderia mallei (Glander) |
5000 |
DOGS AND CATS |
HA test for Canine Parvovirus (CPV) per sample |
500 |
Strip test for Canine Parvovirus (CPV) in dog |
2000 |
ELISA for detection of Antibodies against Rabies virus (For export documentation) |
15000 |
MISCELLANEOUS TESTS (Per Sample) |
Blood test for parasite (Haemoparasites) |
300 |
Complete blood count (Haematological Analyzer) 3 part machine |
700 |
Complete blood count (Haematological Analyzer) 4 part machine |
750 |
White side / surf test for early detection of mastitis per sample |
300 |
Fecal examination for parasites ova detection |
300 |
Fecal examination for parasites Egg per gram (EPG) |
1500 |
Fecal examination for parasites (Floatation technique) |
600 |
Fecal examination for parasites (Sedimentation technique) |
600 |
Skin scrapping for detection of ectoparasites |
300 |
Skin scrapping for detection of fungus |
2000 |
pH of water |
1000 |
Serum Electrolytes |
|
Total serum / plasma Sodium |
300 |
Total serum / plasma Potassium |
300 |
Total serum / plasma Calcium |
300 |
Total serum / plasma Chloride |
300 |
Total serum / plasma protein |
300 |
Total serum / plasma Phosphorus |
300 |
Liver Function Test (LFT) |
|
ALP |
1000 |
ALT |
AST |
Total Bilirubin |
Renal Function Test |
Serum / plasma Creatinine |
700 |
Blood Urea Nitrogen |
- Cost of additional services i.e. preparation of any product, visit of farm (TA/DA, Transportation charges, etc), etc., will be charged separately as per requirement.
- Lab animals are also available on cost basis.
Scope of ISO 17025 testing
Sr. # |
Materials/Products tested |
Types of test/Properties measured |
Range of measurement |
Minimum detection limit |
Uncertainty of Measurement |
Standard |
-
|
Serum |
ELISA for detection of antibodies against IBD |
0.25-9.0
(CPC value) |
0.15 |
0.05 |
Kit Method (Synbiotic, USA 2016) |
-
|
Serum |
ELISA for detection of antibodies against MG |
0.25-9.0
(CPC value) |
0.15 |
0.05 |
Kit Method (Synbiotic, USA 2016) |
-
|
Serum |
ELISA for detection of antibodies against MS |
0.25-9.0
(CPC value) |
0.15 |
0.05 |
Kit Method (Synbiotic, USA 2016) |
-
|
Serum |
Haemagglutination Inhibition Test for Avian Influenza (AI) H5 |
3.0-1024
(HI Titre value) |
2.0 |
0.24 |
OIE (Manual updated 2019) |
-
|
Serum |
Haemagglutination Inhibition Test for Avian Influenza (AI) H7 |
3.0-1024
(HI Titre value) |
2.0 |
0.24 |
OIE (Manual updated 2019) |
-
|
|
Haemagglutination Inhibition Test for Avian Influenza (AI) H9 |
3.0-1024
(HI Titre value) |
2.0 |
0.24 |
OIE (Manual updated 2019) |
-
|
Serum |
ELISA for detection of antibodies against Rabies |
0.25-9.0
(CPC value) |
0.15 |
0.05 |
Kit Method (Synbiotic, USA 2016) |
-
|
Serum |
ELISA for Foot & Mouth Disease (FMD) |
0.25-9.0
(CPC value) |
0.15 |
0.05 |
Kit Method (Prionics, USA 2016) |
-
|
Serum |
ELISA for detection of antibodies against Brucella |
0.25-9.0
(CPC value) |
0.15 |
0.05 |
Kit Method (IDEXX, USA 2016) |
-
|
Serum |
Rose Bengal Test (RBT) |
Qualitative test |
Not applicable |
Not applicable |
OIE Manual Updated 2019 |
-
|
Water |
pH of water |
3:00-10:00 |
2:00 |
0.156 |
AOAC /2016, 20th Edi, Method # 973.41 |
-
|
Equine Species |
Mallein Test |
Qualitative test |
Not applicable |
Not applicable |
OIE Manual Updated 2019 |
Teaching, Research & Development
UDL faculty is also involved in teaching, research & development. A number of research projects have been successfully completed and few are still in process.
|
S# |
Title of Project |
Source of funding |
Amounts of funds (Rs) in Million |
1. |
Preparation of highly specific ELISA Kit on Recombinant outer membrane protein (OMP) of Pasteurella multocida B2 to detect Post-vaccination immune response |
HEC, Pakistan
Under TDF |
9.544 |
2. |
Preparation of live genetically engineered avirulent vaccinal strains derived from local highly pathogenic isolates of P. multocida B:2, using gene deletion method and evaluation of the vaccine by inoculating in mice and calves |
HEC, Pakistan
Under NRPU |
6.714 |
3. |
Hydrogen peroxide based product CareOx35 effect on the health of dairy cows |
Dexon |
0.9 |
4. |
Vaccine Efficacy Testing |
ICI |
1.3 |
5. |
Effect of Different antibiotics and steroidal Residues in Meat of Chicken and their effect on Humans in Punjab |
National Industrial funds |
|
6. |
Epidemiology and Characterization of Bacterial Pathogens Associated with Epizootic Ulcerative Syndrome (EUS) and Motile Aeromonas Septicemia (MAS) from Pakistani Farmed Fish |
National (PARC) |
|
7. |
Molecular Characterization of Bovine Pestivirus isolates from dairy animal of Pakistan and their use in diagnostic, biosecurity and biological development to control disease in Pakistan herds |
National (HEC) |
6.022 |
8. |
Comparative transcriptome analysis of innate and adaptive immune cells during SARS-CoV-2 infection |
International (Qatar) |
5.5 |
Human Resource Development
UDL is playing a vital role in human resource development through organizing workshops, providing trainings, refresher courses and diploma courses for UVAS staff, students, farmers, lab and field professionals and personnel from sister institutions of the country.
International Linkages
Sr. No. |
University / College |
Country |
Nature of Cooperation |
1 |
Division of Lab Animal Medicine, David Geffen School of Medicine, University of California, Los Angeles, CA |
USA |
Faculty development in Lab Animal Facility Management |
2 |
Department of Pathobiology, The University of Connecticut, Connecticut |
USA |
Collaborative Research & Faculty Development |
3 |
Institute of Animal Health, Compton |
UK |
- do - |
4 |
Animal Diagnostic Laboratory, PennState University |
USA |
- do - |
5 |
Atlantic Veterinary College, University of Prince Edward Island |
Canada |
Faculty Development |
6 |
Agriculture & Agri Food Cooperation (AAFC) |
Canada |
- do - |
7 |
University of Qatar |
Qatar |
Collaborative research |
8 |
Vaccine and Infectious Disease Organization- International Vaccine Center (VIDO-InterVac) |
Canada |
Collaborative research |
Clients
- K & Ns Poultry Farms, Raiwind, Lahore
- Supreme Farms, Lahore-Sheikhupura Road
- The Livestock Farm, Rakh Dera Chahl, Lahore
- RVFC (Mona depot, Abbotabad, Sahiwal etc.)
- The Governor House, Lahore
- The Zoo, Lahore
- The Livestock Farms of the Punjab
- The Clinics of UVAS, Lahore.
- The Pets Clinics, Lahore
- The Camel Owners, Lahore
- The Kennel Club, Lahore
- National Feed Pvt. Ltd, Raiwind, Lahore
- The Race Course Club, Lahore.
- The Rangers Lahore
- The Police Line, Lahore
- Rana Nisar Stud Farm, Phool Nagar, Pattoki
- Munir Poultry Farm, Gujranwala
- Idara-e-Kissan
- The Crystal Feed Industry, Multan
- Noor Stud Farm, Sharif City, Raiwind Road
- Other Poultry Farmers of Punjab province
- Private Veterinary Clinics
Location
University Diagnostic Lab (UDL), University of Veterinary and Animal Sciences – UVAS, Syed Abdul Qadir Jillani (Out Fall) Road, 54000 Lahore – Pakistan
Regular Business Hours
Monday – Friday
8: 00a.m. - 4:00 p.m.
Parking Temporary parking for UDL clients on four wheels is available in front of UDL. For motor bikes, in the parking stand on main outfall road. |
