Lecturer
Candidate for Diplomate American College of Theriogenologists, University of Florida, USA
PhD (Animal Molecular and Cellular Biology), University of Florida, USA
MPhil (Theriogenology), University of Veterinary and Animal Sciences, Lahore
Doctor of Veterinary Medicine (DVM), College of Veterinary and Animal Sciences, Jhang
Biography
Ali Husnain is a native of a small village near Gojra, central Punjab, Pakistan. He received his Doctor of Veterinary Medicine (bronze medalist) from the College of Veterinary Medicine in Jhang in 2011. He moved to Lahore and began his Master of Philosophy program with Dr. Nasim Ahmad at the University of Veterinary and Animal Sciences. His thesis focused on manipulating progesterone concentrations after artificial insemination and its effects on the pregnancy rate in dairy buffalo. He became a Teaching Assistant in the Department of Theriogenology at UVAS for nine months and was promoted to a regular faculty position in 2014. Aside from his teaching and research responsibilities, he served as a reproductive consultant on commercial dairy farms and at the University dairy. In 2017, he was awarded a scholarship from the Fulbright Commission of the United States to pursue his Ph.D. degree under the mentorship of Dr. José Eduardo P. Santos at the University of Florida. His Ph.D. program focused on cellular and molecular mechanisms of endometritis-induced compromised fertility in dairy cows. His long-term goals include researching reproductive immunology and conceptus development in dairy animals.
Ali Husnain has expertise in reproductive ultrasonography, hormonal assays (progesterone and estradiol), immunohistochemistry, flow cytometry, gene expression through a real-time polymerase chain reaction, transcriptomics, quantitative metabolomics, and statistical analyses. He completed his two years residency program in comparative reproduction under the mentorship of Dr. Klibs Galvao from the University of Florida. He is a member of the national and international societies related to dairy animal production, nutrition, and reproduction. His vision is to launch the Theriogenology residency program in the Department and strengthen the Progeny Testing Program (PTP) in buffaloes on a community cooperative model nationally.
Area of Interest
- In-vitro embryo production
- Uterine environment and conceptus development
- Reproductive immunology
- Transition period, and nutrition
Publications
- Husnain, A., U. Arshad, M. B. Poindexter R. Zimpel, M. N. Marinho, C. Perdomo, P. Fan, K. C. Jeong, C. D. Nelson, I. M. Sheldon, J. J. Bromfield, and J. E. P. Santos. 2022. Induced endometritis in early lactation compromises production and reproduction in dairy cows. J. Dairy Sci. 105:
- Poindexter, M. B., R. Zimpel, A. Vieira-Neto, A. Husnain, A.C.M. Silva, A. Faccenda, A. Sanches de Avila, P. Celi, C. Cortinhas, J.E.P. Santos, and C.D. Nelson. 2022. Effect of prepartum source and amount of vitamin D supplementation on lactation and reproductive performance of dairy cows. J. Dairy Sci. 105:
- Poindexter, M. B., R. Zimpel, A. Vieira-Neto, A. Husnain, A.C.M. Silva, A. Faccenda, A. Sanches de Avila, P. Celi, C. Cortinhas, J.E.P. Santos, and C.D. Nelson. 2022. Effect of source and amount of vitamin D on serum mineral concentrations and mineral 13 retention in dairy cows. J. Dairy Sci. 105:
- Zimpel, R. K. V. Almeida, M. Nehme Marinho, A. Revilla Ruiz, M. C. Perdomo, M. B. Poindexter, Husnain, A. Vieira-Neto, A. C. M. Silva, U. Arshad, C. D. Nelson, and J. E. P. Santos. 2021. Prepartum level of dietary cation-anion difference fed to nulliparous cows: lactation and reproductive responses. J. Dairy Sci. 104:12580-12599. https://doi.org/10.3168/jds.2021-20486
- Zimpel, M. Nehme Marinho, R. K. V. Almeida, A. Revilla Ruiz, M. C. Perdomo, M. B. Poindexter, A. Vieira-Neto, Husnain, U. Arshad, C. D. Nelson, and J. E. P. Santos. 2021. Prepartum level of dietary cation-anion difference fed to nulliparous cows: acid-base balance, mineral metabolism, and health responses. J. Dairy Sci. 104:11699-11714. https://doi.org/10.3168/jds.2021-20485
- Vieira-Neto, A. M. B. Poindexter, M. Nehme Marinho, R. Zimpel, Husnain, A. C. M. Silva, J. G. Prim, C. D. Nelson, and J. E. P. Santos. 2021. Effect of source and amount of vitamin D on function and mRNA expression in immune cells in dairy cows. J. Dairy Sci. 104:10796-10811. doi.org/10.3168/jds.2021-20284
- Sarwar Z., M. Saad, M. Saleem, Husnain, A. Riaz, N. Ahmad. 2020. Effect of follicle size on oocytes recovery rate, quality, and in-vitro developmental competence in Bos indicus cows. Anim Reprod. 17(3):e20200011. doi.org/10.1590/1984-3143-AR2020-0011
- Sarwar Z., M. Sagheer, F. Sosa, M. Saad, M. Hassan, Husnain, U. Arshad. 2020. Meta-analysis to determine the effects of superstimulation with FSH in progestin-primed environment on in-vitro embryo production using ovum pick-up in Bos taurus cows. Anim. Reprod. Sci. 221:106590. doi: 10.1016/j.anireprosci.2020.106590.
- Husnain, A., and J. E. P. Santos. 2019. Meta-analysis of the effect of prepartum protein on performance in dairy cows. J. Dairy Sci. 102:9791–9813. https://doi.org/10.3168/jds.2018-16043
- Saad, M., Z. Sarwar, M. Saleem, U. Arshad, M. Shahzad, M. H. Mushtaq, Husnain, A. Riaz, and N. Ahmad. 2019. Effect of plasma progesterone on oocyte recovery, oocyte quality, and early in-vitro developmental competence of embryos in Bos indicus dairy cows. Anim. Reprod. Sci. 202:80–86. https://doi.org/10.1016/j.anireprosci.2019.02.001
- Shahzad, A.H., A. Sattar, Husnain, I. Ahmad, N. Ahmad, D. Nak and Y. Nak. 2019. Synchronization and resynchronization as a novel approach for improving the reproductive performance of postpartum dairy cows. Pakistan J. Zool. 51(2):511-520. http://dx.doi.org/10.17582/journal.pjz/2019.51.2.511.520
- Khan, A. S., M. S. Haider, M. Hassan, Husnain, M. R. Yousuf, and N. Ahmad. 2018. Equine chorionic gonadotropin (eCG) enhances reproductive responses in CIDR-EB treated lactating anovular Nili-Ravi buffalo during the breeding season. Anim. Reprod. Sci. 196:28–34. https://doi.org/10.1016/j.anireprosci.2018.06.012
- Riaz, U., M. Hassan, Husnain, M. I. Naveed, J. Singh, and N. Ahmad. 2018. Effect of timing of insemination in relation to onset of standing estrus on pregnancy per AI in Nili-Ravi buffalo. Anim. Reprod. 15:231-1235. http://dx.doi.org/10.21451/1984-3143-AR2017-0015
- Arshad, U., A. Qayyum, M. Hassan, Husnain, A. Sattar, and N. Ahmad. 2017. Effect of resynchronization with GnRH or progesterone (P4) releasing device (CIDR) on cumulative pregnancy and embryonic losses in CIDR-GnRH synchronized Nili-Ravi buffaloes. Theriogenology 103:104-109. https://doi.org/10.1016/j.theriogenology.2017.07.054
- Hassan, M., Husnain, M. I. Naveed, U. Riaz, and N. Ahmad. 2017. Effect of Ovsynch vs. Prostaglandin F2α protocol on oestrus response, ovulation rate, timing of ovulation, and pregnancy per AI in Sahiwal cows. Anim Sci J. 88(3):445-450. https://doi.org/10.1111/asj.12661
- Haider, M.S., M. Hassan, A. S. Khan, Husnain, M. Bilal, J. R. Pursley, and N. Ahmad. 2015. Effect of timing of insemination after CIDR removal with or without GnRH on pregnancy rates in Nili-Ravi buffalo.2015. Anim. Reprod. Sci. 163:24-29. https://doi.org/10.1016/j.anireprosci.2015.09.010
- Yousuf, M. R., J. P. N. Martins, Husnain, U. Riaz, H. Riaz, A. Sattar, K. Javed, and N. Ahmad. 2015. Effect of oestradiol benzoate on oestrus intensity and pregnancy rate in CIDR treated anoestrus nulliparous and multiparous buffalo. Anim. Reprod. Sci. 159:104-108. https://doi.org/10.1016/j.anireprosci.2015.06.003
- Ahmad, N., E. Ahmad, Z. Naseer, U. Riaz, and A. Husnain. 2013. Effect of improving reproductive management on dairy farm economics. Buffalo Bulletin 32(Special 2): 464-467.
- Husnain, A., U. Arshad, M.B. Poindexter, R. Zimpel, E. Schmitt, M.C. Perdomo, M.N. Marinho, K.C. Jeong, J.J. Bromfield, and J.E.P. Santos. 2022. Induced endometrial inflammation compromises conceptus development and growth. J. Dairy Sci. 105 (Suppl. 1); Oral
- Husnain, A., U. Arshad, M.B. Poindexter, R. Zimpel, M.N. Marinho, M.C. Perdomo, P. Fan, K.C. Jeong, C. D. Nelson, J.J. Bromfield, and J.E.P. Santos. 2022. Induced endometrial inflammation impacts production and reproduction in dairy cows. J. Dairy Sci. 105 (Suppl. 1); Poster
- Nehme, M. M., M. C. Perdomo, B. S. Simões, A. Husnain, U. Arshad, C.C. Figueiredo, P.M. Peixoto and J. E. P. Santos. 2022. Lactation performance in dairy cows supplemented with microbial additives. J. Dairy Sci. 105 (Suppl. 1); Poster
- Arshad, U., A. Husnain, M.B. Poindexter, R. Zimpel, M.C. Perdomo, and J.E.P. Santos. 2022. Rumen-protected choline (RPC) influences hepatic metabolism during induction of fatty liver. J. Dairy Sci. 105 (Suppl. 1); Poster
- Arshad, U., A. Husnain, M.B. Poindexter, R. Zimpel, and J.E.P. Santos. 2022. Rumen-protected choline (RPC) reduces hepatic triacylglycerol content by increasing hepatic triglyceride-rich lipoprotein secretion. J. Dairy Sci. 105 (Suppl. 1); Oral
- Husnain A., M. Sagheer, U. Arshad, K.N. Galvão and J.E.P. Santos. 2020. Meta-analysis of the effect of antibiotics and prostaglandin F2α for the treatment of endometritis in dairy cows. J. Dairy Sci. 104 (Suppl. 1); Oral
- Husnain A., and J. E. P. Santos. Meta-analysis of the effects of prepartum dietary metabolizable protein (MP) on performance of dairy cows. J. Dairy Sci. 102 (Suppl. 1); Poster
- Poindexter M. B., A. Vieira-Neto, A. Husnain, R. Zimpel, A. Faccenda, A. Sanches de Avila, A. Silva, P. Celi, C. Cortinhas, J. E. P. Santos and C. D. Nelson. 2019. Effects of dose and source of vitamin D on mineral homeostasis and performance in transition dairy cows. J. Dairy Sci. 102 (Suppl. 1); Oral
- Zimpel R., M. B. Poindexter, A. Vieira-Neto, A. Husnain, S. Buoniconti, P. Celi, C. Cortinhas, C. D. Nelson, and J. E. P. Santos. 2019. Effects of dietary vitamin D3 or 25-hydroxyvitamin D3 on mineral metabolism in growing calves. J. Dairy Sci. 102 (Suppl. 1); Poster
- Ahmed, , A. Husnain, A. A. Channa, M. Z. Tahir, H. Rahman, and N. Ahmad. 2018. Automated system is better than visual observation for detecting of estrus in Holstein Friesian cows. J. Dairy Sci. Vol. 101, Suppl. 2; 348-349. ADSA Meeting, June 23-27, 2018 Knoxville, Tennessee. Abstract # 333 Oral
- Ahmad, N., U. Arshad, A. Qayyum, M. Hassan, A. Husnain, and A. S. Khan. Resynchronization with Ovsynch improves pregnancy per AI and reduces embryonic losses in CIDR-GnRH synchronized Nili-Ravi buffalo. Proceeding of 11th World Buffalo Congress, held in Colombia 2016. Oral
- Ahmad, N., A. S. Khan, M. S. Haider, M. Hassan, A. Husnain, and A. Sattar. 2016. Equine chorionic gonadotropin enhances reproductive responses in CIDR-EB-treated lactating Nili-Ravi buffalo. Abstract Book p: 480-481. Proceeding of 18th International Congress on Animal Reproduction (ICAR-2016), held at LE Vinci Tours, France. Oral
- Sattar, A., A. H. Shahzad, A. Husnain, I. Ahmad, and N, Ahmad. 2016. Effect of resynchronization on already synchronized postpartum dairy cows. Abstract Book p: 553. Proceeding of 29th World Buiatrics Congress, held at Dublin Ireland, on 3-8 July 2016. Oral
- Husnain, A., A. Rehman, M. Ahmad, U. Arshad, A. Sattar, and N. Ahmad. 2016. Model for AI decision: Pre-insemination single-time ultrasound scanning improves fertility in bovines. Abstract Book p: 550. Proceeding of 18th International Congress on Animal Reproduction (ICAR-2016), held at LE Vinci Tours, France. Oral
- Husnain, A., U. Riaz, M. I. Naveed, M. Hassan, M. Abdul Sattar, and N. Ahmad. 2015. Post insemination interventions: effect of human chorionic gonadotropin, gonadotropin-releasing hormone, and progesterone on ovulation and conception rates in Nili-Ravi buffaloes. J. Anim. Sci. Vol. 93, Suppl. s3/J. Dairy Sci. Vol. 98, Suppl. 2. Proceeding of Joint Annual Meeting of ADSA and ASAS, held at Orlando Florida USA, on July 12-16, 2015. Oral
- Hassan, M., A. Razzaq, A. Husnain, A. A. Channa, and N. Ahmad. 2015. Estrus synchronization does not improve fertility in chronically repeat breeding Sahiwal cows. J. Vet. Anim. Sci. 5(Suppl. 1). Proceeding of International Symposium on Dairy Animal Reproduction at UVAS, Lahore. Poster
- Husnain, A., and N. Ahmad. Replacement for the commercial dairy farm: Intensive breeding program for pre-pubertal buffalo heifers. J. Vet. Anim. Sci. 5(Suppl. 1). Proceeding of International Symposium on Dairy Animal Reproduction at UVAS, Lahore. Poster
- Husnain, A., A. Rehman, and M. A. Sattar. 2015. A Case Report; Fetal mummification accompanied with viable kids at full ferm in Teddy x Beetal Doe. J. Vet. Anim. Sci. 5(Suppl. 1). Proceeding of International Symposium on Dairy Animal Reproduction at UVAS, Lahore. Poster
- Riaz, U., A. Husnain, M. I. Naveed, U. Farooq, and A. Riaz. 2015. The way to preserve the genome of the valuable animal: epididymis gamete retrieval in slaughtered ram and its post-thaw evaluation. J. Vet. Anim. Sci. 5(Suppl. 1). Proceeding of International Symposium on Dairy Animal Reproduction at UVAS, Lahore. Poster
- Abbas, S. A. Husnain, M. Hassan, and N. Ahmad. 2015. Effect of timing of AI on pregnancy in estrus synchronized Nili-Ravi Buffaloes: A Preliminary Study. J. Vet. Anim. Sci. 5(Suppl. 1). Proceeding of International Symposium on Dairy Animal Reproduction at UVAS, Lahore. Oral
- Naveed, M. I., A. Husnain, U. Riaz, M. Hassan, A. Sattar, and N. Ahmad. 2014. Equine chorionic gonadotropin improves follicular dynamics, estrus expression, ovulation, and pregnancy rate in CIDR-based estrus synchronization protocol in Nili-Ravi buffalo. J. Anim. Sci 92(E-Suppl. 2)/J. Dairy Sci. 97(E-Suppl. 1): 262-262. Proceeding of the joint annual meeting of ASAS/ADSA held at Kansas City Missouri, USA. Oral
- Ahmad, N., U. Riaz, A. Husnain, M. Hassan, M. I. Naveed, and A. Sattar. 2014. Effect of timing of artificial insemination and ovulation in relation to onset of standing heat on pregnancy rate in dairy buffaloes. Advances in Animal Bio Sciences 5(2):261. Proceedings of International Cow Fertility Conference, held at Westport Ireland on 18-21 May 2014. Oral
- Ahmad, N., E. Ahmad, Z. Naseer, U. Riaz, and A. Husnain. 2014. Effect of improving reproductive management on dairy farm economics. Buffalo Bulletin32(2):464-467. Proceedings of 10th World Buffalo Congress held at Phuket, Thailand. Oral
Research Grants, Contracts, and Gifts
Awarded (Total PKR 16.23 M, US $00, as PI and Co-PI)
2022 PKR 14.701 M, Punjab Agriculture Research Board, Mitigating the negative consequences of environmental stressors by supplementing lipids and production of omega-3 fatty acid-enriched milk. Submitted as PI
2015 PKR 1.51 M, Malaysian Palm Oil Council. Effect of Palm Kernel Cake (PKC) feeding on production efficiency of dairy cows. Approved as Co-PI
2015 PKR 19.00 M, Punjab Agriculture Research Board. Novel approaches for enhancing artificial insemination outcomes in buffaloes. Denied as Team Scientist
2015 PKR 33.80 M, Punjab Agriculture Research Board. Advance biomarkers to identify infertility and modern strategies for improving reproductive efficiency in food-producing animals. Denied as Team Scientist
2015 US $ 157310, Pak-US Technology Cooperation Phase VI. Virtual vet: Smart livestock monitoring and management System. Denied as Co-PI
2015 PKR 0.36 M, University of Veterinary and Animal Sciences, Lahore. Request approval and provision of items (medicines and instruments) for Theriogenology clinics (A & C block) at Ravi campus, Pattoki. Approved as PI
2014 PKR 0.36 M, University of Veterinary and Animal Sciences, Lahore. Development and modification in the animal house for undergraduate teaching (DVM 7th and 9th semester) at Ravi campus, Pattoki Approved as PI
Achievements/Honors/Awards
2021 Travel awards as a graduate scholar to attend the Dairy Cattle Reproduction Council (DCRC) annual meeting, held on November 9-11, 2021. at the Loews Kansas City Hotel. Awarded $1500 from the DCRC program for the graduate scholar.
2021 Awarded the complimentary registration to attend the Society for Theriogenology (SFT) Conference virtual meeting, held on July 21-24, 2021. Awarded $250 from the residency program of Veterinary College of the University of Florida.
2018 Travel sponsorship to participate in Fulbright Enrichment Seminar, May 10-13, 2018, Nashville, Tennessee. Organized by the Fulbright Commission of the United States, where over 100 Fulbright scholars gather from different counties.
2018 Travel award the support to participate in AABP Edwin Robertson Beginning Embryo Transfer Seminar, held on August 12-16, 2018, at Virginia Tech, VA. Awarded $2000 registration fee along with the travel and lodging for five days by United States Livestock and Genetic Export (USLGE), Madison, USA.
2017 Travel sponsorship to participate in the Fulbright Pakistan Orientation Seminar, November 2-5, 2017, University of Wisconsin, Madison. Organized and sponsored by the Fulbright Commission of the United States. Organized by the Fulbright Commission of the United States, where over 100 Pakistani Fulbright scholars gather.
2017 Travel award to attend the American Embryo Transfer Association (AETA) and Canadian Embryo Transfer Association (CETA/ACTE) joint convention, held on October 26–28, 2017, in Orlando, Florida. Awards for complimentary registration, travel, and lodging for three days by AETA.
2017 Travel award to attend the Word Dairy Expo 2017, held on October 2-7, 2017, at Madison, Wisconsin. Awarded travel support and lodging for six days by United States Livestock and Genetic Export (USLGE), Madison, USA.
2017 Travel award for participating in International Dairy Short Course held on October 2-4, 2017, in Madison, Wisconsin from United States Livestock and Genetic Export (USLGE), Madison, USA. Awarded travel support and lodging for three days.
2017 Fully funded Ph.D. Scholarship from the Fulbright Commission of the United States under the Animal Molecular and Cellular Biology (AMCB) program in the Department of Animal Sciences at the University of Florida. Awards of $260,392 cover tuition fees, living allowance, and health insurance from 2017 - 2022.
2015 Second Position in a Poster Competition on ‘replacement for the commercial dairy farm: an intensive breeding program for pre-pubertal buffalo heifers’ in International Symposium on Dairy Animal Reproduction (ISDAR) held on March 30 – 31, 2015, at UVAS, Lahore. Awarded Cash prize and certificate.
2011 Recipient of Bronze Medal, holding the third position in DVM 2006-11 batch at UVAS Lahore.
2011 First Position in the final semester of DVM Internship Program 2011. Awarded with a cash prize and a letter of appreciation.
2008 Selected for Research Trainee on merit-based in Pakistan Dairy Development Company (PDDC) from August to October 2008.
2006 - 11 Awarded for Merit Scholarship throughout DVM program at CVAS Jhang.