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University Diagnostic Laboratory
 
 
 
 

   Introduction

Initially the Lab started with the processing / analysis of less than 500 samples in 1998 that raised upto 5000 samples in one year after the establishment of University of Veterinary and Animal Sciences (UVAS), Lahore since June 8, 2002. The number of cases multiplied day by day and now has been raised to over 12000 upto December, 2007 per year.

University Diagnostic Lab has got almost all those diagnostic facilities requisite for routine monitoring of the livestock herds, poultry flocks, farms, hatcheries, and feed mills to formulate preventive measures for avoiding possible mortality losses of their animals in particular and economical losses to the industry in general. Besides conventional microbiological, serological, coprological, hematological tests and postmortem examinations, many new modern diagnostic facilities like Polymerase Chain Reaction (PCR) for various diseases have been optimized. The diagnostic facilities at UDL were strengthened by the project of about 40 million funding from Higher Education Commission for early / accurate diagnosis and for advanced research in veterinary diagnostics

 Aims & Objectives

An early diagnosis usually leads to accurate and successful treatment of the diseased animal. With this objective in mind University Diagnostic Lab is serving the nation by rendering diagnostic services to the livestock and poultry farmers and also to the pet owners. University Diagnostic Lab has got almost all those diagnostic facilities requisite for routine monitoring of the livestock herds, poultry flocks, farms, hatcheries, and feed mills to formulate preventive measures to avoid losses owing to possible morbidity and mortality of their animals in particular and economical losses to the industry in general. Besides conventional microbiological, serological, coprological, hematological tests and postmortem examinations, many new diagnostic facilities are introduced for the confirmation of all those diseases of livestock and poultry, which are economically devastating to the stakeholders of our country. Keeping in view the deficient areas and constraints of the sector, UDL is planning to work on four new upcoming areas viz. HRD (Degree programs, Diploma courses and short trainings in lab diagnostic techniques), Helpline (to guide the farmers, stakeholders and pet owners), Research and Development (developments of kits and reagents that are used in different diagnostic tests), Disease warning and quality assurance (season based early disease warning system) and diseases of fish and wild animals. Pride of serving many national and international organizations that platform for developing linkages and MOUs between UVAS and these institutes.

  Facilities Available

University Diagnostic Lab has got almost all those diagnostic facilities requisite for routine monitoring of the livestock herds, poultry flocks, farms, hatcheries, and feed mills to formulate preventive measures for avoiding possible mortality losses of their animals in particular and economical losses to the industry in general.
In the above-mentioned context UDL is providing high quality diagnostic services to the farmers at very nominal rates (list appended in advertisement section), which are almost 100 per cent less than the rates charged by privately owned diagnostic labs. Moreover, growth and success of the UDL can be observed from the trust shown by our clients whereas, dedication, devotion and commitment of its staff can be ascertained by the fact that the lab processed not more than 1000 samples per annum for the initial five years(1998-2002) but after the establishment of UVAS, has processed over 5000 samples in year 2003 only. Presently this number has crossed the figure of 10000 samples per annum.
Besides conventional microbiological, serological, carpological, hematological tests and postmortem examinations, many new diagnostic facilities including PCR based molecular biology tests are going to be introduced in the near future for the confirmation of all those diseases of livestock and poultry, which are economically devastating to the stakeholders of our country.

  Tests Offered

Name of The Test

Rupees/Sample

MICROBIOLOGY

Postmortem for poultry

50

Culture sensitivity

100

Viable bacterial count

200

Coliform count

200

Isolation and identification of Salmonella (Species)

300

Isolation and identification of E. coli (Species only)

300

MG culturing and identification

500

Plate exposure for bacteria

150

Antibacterial activity of disinfectant

1000

Antiviral activity of disinfectant

1500

ND and AI isolation and identification

1500

SEROLOGY

Pullorum plate agglutination test

30

MG plate agglutination test

30

MS plate agglutination test

50

Rose Bengal Plate test

25

Brucella Tube Agglutination test

50

HI for ND or AI or EDS

10

HI for IB antigen

35

Agar gel precipitation test (Qualitative)

25

Fluorescent antibody test

300

MISCELLANEOUS TESTS (Per Sample)

Mallein test

30

Tuberculin test

30

Blood test for parasite

25

Complete blood test

100

Urine test (WBC. pH. Crystals )

100

White side test

10

Fecal examination for parasites ova

20

Skin scrapping for parasites

20

Ph of water

10

Hardness of water

10

HA test for confirmation Canine Parvovirus (CPV) per sample

100

Addition of New Tests

Sodium
Potassium
Calcium
Lithium
Total Serum / plasma Protein
Total Serum / plasma Calcium
Total Serum / plasma Magnesium
Total Serum / plasma Phosphorus
PCR for diagnosis of Bird flu (H5N1) virus
PCR for diagnosis of Avian Influenza (H9N2) virus
PCR for diagnosis of Avian Influenza (H7N3) virus
PCR for diagnosis of ND virus
PCR for diagnosis of Rabies virus
PCR for diagnosis of Canine Distemper virus
PCR for diagnosis of Canine Parvo virus
PCR for diagnosis of Mycoplasma gallisepticum

  Research Development Projects (Near Completion)

  • Strengthening of facilities, at the University Diagnostic Lab, UVAS, Lahore and Animal House at Bhunikey Campus, UVAS, worth Rs. 38.108 million funded by Higher Education Commission (HEC), Islamabad.

  • Rapid Diagnosis and immunoprophylaxis of acute viral diseases of canine at UDL, UVAS, Lahore, worth Rs. 2.00 million funded by funded by Higher Education Commission (HEC), Islamabad.

  Staff Position

SNAP / NAME / DESIGNATION



DR. MASOOD RABBANI
Ph.D.
Professor / Director
 

DR. TAHIR YAQUB
Ph.D.
Associate Professor
 

MR. AAMIR GHAFOOR BAJWA
M.Sc. (Hons.)
Assistant Professor
 

MR. ALI AHMAD SHEIKH
M.Sc. (Hons.)
Lecturer
 

MR. ARFAN AHMAD
M.Sc. (Hons.)
Lecturer
 

MR. M. ZUBAIR SHABBIR
D.V.M.
Lecturer
 

MS. FARIHA AKHTAR
D.V.M.
Lecturer
 

  PRINCIPAL EQUIPMENT / SALIENT FACILITIES

 

Principal Equipment Salient Features/Services
  • Autoclaves, Hot air oven and Safety Cabinets DNA plus, etc
  • Lab incubators, egg incubators CO2 incubators, etc.
  • ELISA reader,ELISA plate washer, Micro-pipettes, etc.
  • Set of micropipettes, microtitration system
  • Fluorescent microscope, Safety cabinet, incubator
  • Microscope with CCTV
  • Mc Master slide, Microscope
  • pH meter
  • Rotators, multishakers, resonance blenders, sonicators, etc.
  • Biohazard safety cabinet, Refrigerated Centrifuge, Pipettes, Thermocycler, Electrophoresis apparatus, DNA concentrator, Gel documentation system
  • Ultra low freezer, liquid & contrument cittra centrifuge Machine, Distillation plant (Millipore), Hematological analyzer, Chemistry Analyzer, Flame Photometer, Densitometers, Sonicators, virus processing & purification systems, reference balances
     
  • Biosafety, Culture sensitivity, Viable bacterial count (water, feed, feed ingredients, Hatchery fluff, etc.), Coliform count, Isolation and identification of Salmonella (Species), E. coli (Species only)
  • MG culturing and identification, Plate exposure for bacteria, Antibacterial activity of disinfectant, Antiviral activity of disinfectant.
  • ELISA for detection of Antibodies against IBD (Gumboro), Mycoplasma gallisepticum, Mycoplasma synoveae, Fowl Cholera, Chicken Anemia Agent, Inf. Laryngotracheitis, Reovirus, Foot & Mouth Disease Virus, Canine Parvovirus & Canine Distemper
  • HI for Newcastle disease (ND) or Avian Influenza (AI), Egg Drop Syndrome (EDS), Canine Parvovirus, IB (M-41) (D-274)
    (D-1466).
  • Fluorescent antibody test for the diagnosis of canine rabies virus.
  • Blood test for parasite, Skin scrapping for parasites, Fecal examination for parasites ova
  • New castle disease (ND) and Avian Influenza (AI) isolation and identification
  • PCR for the diagnosis of various microbial diseases, Purification of viruses, Cryogenic storage facility of seed cultures of bacteria and viruses., Molecular biological grade DNAs, RNAs and bacterial free double de-ionized distilled water.
  • Complete blood test, Chemical analysis of biological fluid, Electrolyte analysis (Sodium, Potassium, Calcium and Lithium) in Biological fluid

  IMPLEMENTATION OF ISO / IEC 17025

ISO (International Organization for Standardization) and IEC (International Electro-technical Commission) form the specialized system for worldwide standardization. National bodies that are members of ISO or IEC participate in the development of international standard through technical committees established by respective organization to deal with particular fields of technical activity. ISO / IEC 17025 is an international standard developed by CASCO (ISO Committee on Conformity Assessment) and was approved by both bodies ISO and IEC, so called ISO / IEC 17025.

  Implementation of ISO / IEC 17025 at UDL

UDL is testing and disease diagnostic lab and aims to achieve international level quality in testing, at present the Lab is performing its test at 95% of Confidence level. In future it will raise its quality up to 99% of Confidence. More than thirty tests are in the scope of accreditation. To achieve 99% confidence level and conformity of competence the authorities of this Lab decided to implement Lab Management System (ISO / IEC 17025).
Implementation of this standard in the UDL is in process since August 2006. This activity started with the 1st level training of UDL staff to develop awareness toward this standard and now is in last phase of pre-assessment and final accreditation. UDL applied for accreditation to Pakistan National Accreditation Council (PNAC), Islamabad. Initial assessment in this regard has been done by the PNAC and full assessment is expected in mid of 2008.
 

 

 
     

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