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Faculty of Veterinary Science

 
 
Department of Microbiology

Introduction

The department has made significant contributions in the field of conventional diagnostics and vaccines as is evident from the fact that many students both at Ph.D. and Master level had been trained and are serving in various capacities at different private and public establishments of the country. The Department is actively engaged in teaching under- and postgraduate, deficiency and diploma courses. Training workshops for field veterinarians are also being arranged. The Department has produced quite a number of M. Phil. and Ph.D. students in the University. The Department is equipped with modern tools, cell culture lab and lab animal facility.

Staff Position

Chairman

Professors

  1. Prof. Dr. Irshad Hussain (EOL Ex. Pakistan)
  2. Prof. Dr. Aftab Ahmad Anjum

Assistant Professors

  1. Ms. Sameera Akhtar (EOL Ex. Pakistan)
  2. Dr. Jawad Nazir
  3. Shahan Azeem
  4. Dr. Muhammad Nawaz
  5. Dr. Rabia Tanvir

Lecturers

  1. Imran Najeeb
  2. Muhammad Asad Ali
  3. Ms Farah Khan 
  4. Hassaan Bin Aslam 
  5. Ms Rabia Tahir 
  6. Kashif Hanif

HEC Approved Supervisors

Prof. Dr. Khushi Muhammad

Facilities

Principal Equipment Salient Features/Services
  • Autoclave
  • Hot air oven
  • Refrigerated Centrifuge
  • Fluorescent Microscope
  • Inverted Microscope
  • Fluoro Spectrophotometer
  • Class I and II Safety cabinet
  • -70 C Freezer
  • -20 C Freezer
  • Water distillation plant
  • Vertical Electrophoresis
  • Horizontal Electrophoresis
  • Sterilization
  • Sterilization
  • High speed centrifugation at low temperature
  • Fluorescent antibody technique
  • Analysis of live cells/ cell culture
  • Biochemical analysis of samples
  • To provide sterile area for lab work
  • Storage of samples & long preservation of cultures
  • Storage of samples and short preservation of cultures
  • To provide triple distilled water for cell culture & routine lab work
  • To run protein samples
  • To run DNA samples
  • Laboratory animals: Balb C/J mice colony is reared for monoclonal antibody production

Research Projects in Progress

Research is a main tool for raising the standard of life of the public. In UVAS, researchers/teachers conduct research to solve problems of the industries such as poultry, livestock, bioceutics, pharmaceutic, fish, equine and ordinary dairy farmers, milk processing, exporters and importers and government issues related to livestock. In universities every postgraduate research scholar is supposed to solve one problem of the industry/farmers. To minimize the financial consternation of the students, every Ph.D. faculty every year won at least one research grant on competitive basis from funding agencies of the country. On similar basis, following grants were obtained and postgraduate scholars conducted research on the projects and completed their research as required for their postgraduate degree program

S.#

TITLE OF THE PROJECT

COST Rs. (million)

STATUS

1.

Characterization of avian influenza in poultry (funded by Olympia Poultry Breeders-PVT-Ltd,  Big Birds- PVT-Ltd, Avicenna Labs -PVT-Ltd)

0.087 1997

2.

Prevalence of egg drop syndrome virus in commercial poultry (University research promotion fund) 

0.055

2002

3.

Immunobiology of Newcastle disease virus in poultry (University research promotion fund)

0.042   

2000

4.

Preparation and evaluation of Mycoplasma capri vaccines (University research promotion fund)

0.087

2000

5.

Immunoprophylaxis of Haemorrhagic Septicaemia in bovine (University research promotion fund)

0.078

2005

6.

Passive immunization of hydropericardium syndrome infected broilers (University research promotion fund)    

0.028

2004

7.

Immunoprophylaxis of Foot and Mouth Disease in bovines (ALP/PARC funded)   

5.980        

2006

8.

Development of Enzyme Linked Immuno Sorbant Assay (ELISA) Kit for diagnosis of bovine infectious diseases. PCST approved

2.094

2006

9.

Strengthening of University Diagnostic Laboratory at University of Veterinary and Animal Sciences, Lahore and animal house at Bhoneiki Campus, of UVAS, Lahore (Proposal Submitted to HEC)

39.6

2004-2008

10.

Quality Control Laboratory for Testing Livestock and Food of Animal Origin with Reference to World Trade Organization (WTO)

99.998

2004-2007

11

Development of protocols for production and evaluation of hydropericardium syndrome vaccine and immunised yolk/serum

0.0705

2006-2008

12

Isolation and molecular characterization of Shiga Toxin producing E. Coli O157

2.316

2006-2011

13.

Identification of foot and mouth disease virus from clinically infected animals and genotyping of VP 1 gene of FMD type “O” viruses

1.400

2007-2009

14.

Production and evaluation of trivalent inactivated avian influenza vaccine

2.600

2007-2010

15.

Monitoring of chemical adulterants and hygienic status of market milk

0.700

2007-2009

16.

Quality testing of poultry meat and eggs

1.700

2007-2010

17.

Sustainability of Quality Operations Laboratory, UVAS, Lahore

51.55

2008-2009

18

Augmentation of production potential of FMD+HS vaccine and immunoprophylaxis of the diseases in cattle and buffaloes

23.55

2009-2011

 

HUMAN RESOURCE DEVELOPMENT (WORSHPOS):

 

S.#

Title

Duration (Days)

Candidates

Dates

1

Primer designing for microbial identification using PCR

2

20

0911-09

1011-09

2

Advanced Laboratory Techniques

12

7

04052009

16052009

3

Advanced Diagnostic Techniques and Control of Poultry Diseases

6

12

15032010

20032010

4

Basic diseases diagnostic techniques in Livestock and poultry

6

10

05042010

10042010

5

Applied Research on advanced laboratory techniques

4

10

17052010

21052010

6

Molecular techniques (PCR/RT-PCR) for diagnosis of animal microbial diseases

4

23

1611-09

1911-09

7

Monitoring of raw milk for sorbitol non-fermenting E. coli

5

12

1705-10

2105-10

8

Propagation of Newcastle disease virus in chicken embryos and detection of its immunogenic protein using SDS-PAGE

5

30

3105-10
0706-10
1207-10

0406-10
1107-10
1607-10

9

Hygienic Monitoring of Food and Food Products

5

15

0507-10

0907-10

10

Isolation, Screening and Preliminary Characterization of Antimicrobial Metabolites from Food Microbes

5

20

1907-10

2307-10

11

Preparation and Evaluation of Oil based Multivalent Foot and Mouth Disease and Hemorrhagic Septicemia  Vaccine

5

12

0208-10

1108-10

12

Bio-fermentation for Biomass Production of Pasteurella multocida

5

10

2308-10

2708-10

13

Diagnostic approaches towards avian Mycoplasmosis  with collaboration of USA aid project Prof. Dr. Muzaher Hussain Khan

2

24

0601-11

0701-11

 

  Faculty Development

Sr Description of Seminar/ Synopsis / Training Place Date Participated By
1 RT-PCR for diagnosis of FMD virus UVAS Jun 5-10, 06 Aamir Ghafoor
2 Characterization & Management of emerging viral Diseases NIBGE Nov 14-22, 06 M. Imran Najeeb
3 Teachers orientation program UVAS Mar 12-28, 06 M. Imran Najeeb
4 Faculty Development program UVAS Dec 06-Feb 07

Dr. Mansur-ud-Din Ahmad
Dr. Irshad Hussain
Prof. Dr. Aftab Ahmad Anjum

5 Case Teaching Methodology HEC Mar 8-9, 07 Prof. Dr. Aftab Ahmad Anjum
6 Self Assessment as a tool for Quality Assurance UVAS Jun 14, 06

Dr. Mansur-ud-Din Ahmad
Prof. Dr. Aftab Ahmad Anjum

Invited Lectures

Sr

Topic

Delivered By Venue
1 Microscope and Microscopy

Dr. Mansur-ud-Din Ahmad
Jawad Nazir

TOP
2 Spectrophotometry, Chromatography, Lab. Mathematics, Basics of gene cloning, How to make transgenics, and knockouts Dr. Irshad Hussain -do-

New Academic Programs Planned

The proposal for 4 years B.Sc. (Hons.) Microbiology Degree program was initiated and approved by the BOS of the Department and university authorities. The curriculum has been developed and hopefully admission will start in coming academic session.

New Academic Programs Planned

The proposal for 4 years B.Sc. (Hons.) Microbiology Degree program was initiated and approved by the BOS of the Department and university authorities. The curriculum has been developed and hopefully admission will start in coming academic session.

Measure taken to Enhance the Academics

Research activities of the Department are enhanced and regularized. Research in Progress is as under:

  • Advanced molecular biological techniques are being standardized

  • Standardization of ELISA for the detection of antibodies against Hydropericardium syndrome virus ( Intezar Hussain, M.Phil.).

  • Detection of immunogens of HPS virus ( Zafar Iqbal, M.Phil.).

  • Study of immunogenicity of HPS virus on different age groups of broilers (Fariha Akhtar, M.Phil.).

  • Selection of thermostable reconstituted NDV progeny from vaccinal strain (Faiza Ghazanfar).

  • Studies on the factors affecting Pathogenicity of Fowl adenovirus causing Hydropericardium syndrome in broilers ( Shahid Zaman).

  • Electrophoretic profile of E. coli isolated from field ( Kaleem Ullah, M.Phil.).

  • Electrophoretic profile of Staphylococcus aureus from mastitic milk ( M. Zubair Munir, M.Phil).

  • Biological and molecular characterization of indigenous isolate of foot and mouth disease virus
    ( Rashid Munir Khawaja, Ph.D.).

  • Development & evaluation of polyvalent vaccines against AI virus ( T. M. Shaukat, Ph.D.)

Books Published

  • Booklet in Urdu, Poultry farming and Biosecurity, By Dr. M. Akram, Dr. M.A. Muneer, S. Hanif and S. Azeem, 2006.

  • Booklet in Urdu, "Basic Microbiology", by Dr. M.D. Ahmad, A.A. Anjum, and M.I. Najeeb through ICE&E, UVAS, Lahore 2006

Monograph

Monograph on "Recent Developments in Avian Influenza in Pakistan" Monograph synopsis approved by Higher Education Commission, Islamabad vide Ref. No. 20-84 / HEC / Media 2005 / 572 dated March 25, 2006.

Work is in progress for the preparation of PC-1s for:

  1. Institute of Pathobiology

  2. Strengthening / Upgradation of Department

  3. B.Sc. (Hons.) Microbiology Degree Program

Research Priorities

 
  • Isolation and identification of the causative agents of the newly emerging microbial diseases of livestock and poultry.

  • Preparation and evaluation of biologics (vaccines, antimicrobial factors, diagnostic reagents, etc.)

  • Qualitative and quantitative detection of mycotoxins and measures to overcome mycotoxicosis in animals.

  • Physico-chemical factors affecting the survival of microbial pathogens (Biosecurity).

  • Immunogenetics, Immunotherapy, Immunomodulation, Immunoprophylaxis, Immunoregulations and Immunodiagnosis of various diseases.

 
 

Department of Microbiology